In this contribution, we attempt to define fast cysteine bioconjugation, in contrast the traditional modifying reactions that are kinetically slow. A conjugation reaction can allow facile quantitative labeling of proteins with minimized side and much simplified purification. substantial collection novel have been developed towards end some displaying rates over 103 M−1 s−1. This Concept article...

We present an efficient method to synthesize three new asymmetric trimethine cyanine dyes containing only one carboxylic acid group for bioconjugation. Two of them have better protein labeling performance than other conventional cyanine dyes due to their particular structure design.

Combining N-heterocyclic carbenes with gold nanoparticles provides an ultrabright and fragment-free ionization platform for laser-desorption mass spectrometry. In their Research Article (e202219182), David M. Jenkins, Jon P. Camden, co-workers show that NHC-functionalized can “give wings” in the spectrometer, enabling next-generation applications of barcoding, data storage, bioconjugation react...

We present here a new and alternative method that uses a Fluorescence Plate Reader in a different approach, not to study protein-protein interactions, but to evaluate the efficiency of the protein bioconjugation to quantum dots (QDs). The method is based on the QDs' native fluorescence and was successfully tested by employing two different QDs-proteins conjugation methodologies, one by promotin...

[(18)F]-5-Fluoro-5-deoxyribose ([(18)F]-FDR) conjugates much more rapidly than [(18)F]-FDG under mild reaction conditions to peptides and offers new prospects for mild and rapid bioconjugation for fluorine-18 labelling in PET imaging.

A facile methodology for light-triggered release of thiols under mild conditions is presented, which can be utilized for in situ bioconjugation with protein and quantum dot nanoparticles (QDs) efficiently.

The advent of antibody-drug conjugates as pharmaceuticals has fuelled a need for reliable methods of site-selective protein modification that furnish homogeneous adducts. Although bioorthogonal methods that use engineered amino acids often provide an elegant solution to the question of selective functionalization, achieving homogeneity using native amino acids remains a challenge. Here, we expl...

Copper(I)-catalyzed 1,3-dipolar cycloaddition of azides and terminal alkynes (CuAAC), better known as "click" reaction, has triggered the use of 1,2,3-triazoles in bioconjugation, drug discovery, materials science and combinatorial chemistry. Here we report a new series of water-soluble catalysts based on N-heterocyclic carbene (NHC)-Cu complexes which are additionally functionalized with a sul...

Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which...