The controversy regarding the safety of saccharin for human consumption started shortly after its discovery over 100 years ago and has yet to subside appreciably. The consumption of saccharin, particularly in North America, began to escalate when the U.S. Food and Drug Administration set new standards of identity which allowed foods containing artificial sweeteners to be promoted as "nonnutriti...

Branchiura sowerbyi (Oligochaeta; Naididae; Rhyacodrilinae) is an oligochaete with great potential to be used as a testspecies in toxicology bioassays. Since 1950, its life cycle has been studied and nowadays it is well described in the literature. B. sowerbyi has a cosmopolitan distribution and can be found in places where Tubifex tubifex (normally used in toxicity bioassays) does not occur, e...

Bradley N. Johnson Tami L. Stubbs Land Management and Water Conservation Research Unit, USDA Agricultural Research Service, 215 Johnson Hall, Washington State University, Pullman, WA, 99164-6421 Pseudomonas fluorescens strain D7 (P. f. D7; NRRL B-18293) is a root-colonizing bacterium that inhibits downy brome (Bromus tectorum L. BROTE) growth. Before commercialization as a biological control ag...

Cell secretome analysis has gained increasing attention towards the development of effective strategies for disease treatment. Analysis of cell secretome enables the platform to monitor the status of disease progression, facilitating therapeutic outcomes. However, cell secretome analysis is very challenging due to its versatile and dynamic composition. Here, we report the development of two imm...

Determination of dioxin-like compounds utilizing in vitro bioassays such as ethoxyresorufin-O-deethylase (EROD) or chemical-activated luciferase expression (DR-CALUX) is an important tool to evaluate their Ah receptor-mediated toxic effects. The aim of this study is to describe advantages and limitations of these bioassays using rat hepatoma (H4IIE) wildtype and recombinant H4IIE cells in a 96 ...