Immune genes of the major histocompatibility complex (MHC) are among the most polymorphic genes in the vertebrate genome. Due to their polymorphic nature, they are often used to assess the adaptive genetic variability of natural populations. This study describes the first molecular characterization of 13 partial MHC class IIB sequences from three European ranid frogs.  The utility of previously...

Alpha-amylase is widely used as an industrial enzyme, and a therapeutic target for which inhibitors are designed. Organic solvents are used to dissolve various compounds that would be studied as moderators of alpha-amylases, but they could themselves affect enzyme activity and stability. Methanol, ethanol and propanol are simple alcohols that may be commonly used to this end, and their effect h...

In the present work, the interaction ofFe3O4@CaAl LDH@ Lamivudine with human serum albumin (HSA) was investigated by applying UV–vis and fluorescence spectra. The nanocomposite was quenching the natural fluorescence of HSA, which was indicated the static quenching mechanism. The consequences demonstrated that this nanocomposite can strongly bind to HSA molecules. According to fluorescence quenc...

This paper is designed to examine the binding behavior of Coumarin with bovine -casein (βCN) through fluorescence spectroscopy and molecular modeling techniques. The data analysis on fluorescence titration experiments at various temperatures represents the enthalpy driven nature for the formation of Coumarin–βCN complex and the prevailed role of hydrogen bonds and van der Waals interactions in...

the melanocortin-1 receptor mc1r is encoded by the extension locus, playing a fundamental role in the determination of coat color in a number of mammalian species. however, so far there has been no report regarding the single nucleotide polymorphisms (snps) of the mc1r promoter region and the potential association of its snps with coat color in sheep (ovis aries). throughout the present study, ...

alpha-amylase is widely used as an industrial enzyme, and a therapeutic target for which inhibitors are designed. organic solvents are used to dissolve various compounds that would be studied as moderators of alpha-amylases, but they could themselves affect enzyme activity and stability. methanol, ethanol and propanol are simple alcohols that may be commonly used to this end, and their effect h...

Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic feature...

KAl(SO4)2.12H2O is found to efficiently and heterogeneously catalyze the one-pot three-component reaction of 2-(nitromethylene)imidazolidine, malononitrile and aldehydes under mild conditions to afford the corresponding tetrahydroimidazo[1,2-a]pyridine in good yields and short reaction times. Docking  study  of  some  compounds  in  the  active  site  of  α-glucosidase demonstrated  that  these...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

Because of participation in many aspects of human life, and due to oxidation-sensitive characteristics of dopamine (DA) and arachidonoyl dopamine (AA-DA), the necessity of biocompatible carrier to keep them against oxidation is of importance. In this work, we explored the putative binding sites of DA and AA-DA to -lactoglobulin (BLG) as potent carrier. Docking results identified the binding si...