Plant tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Combination of 6-Benzylaminopurine (BAP) and other plant growth regulators like 1-Naphthaleneacetic acid (NAA) or Indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) was used in the most of the research in tissue culture. The study was carried out to investigate the optimization of...

Micropropagation is an effective way to preserve the gene pool of threatened plants. This study devoted mathematical modeling nutrient media and effect mT (meta-topoline) on multiplication shoots Hedysarum grandiflorum, Hyssopus cretaceus, Matthiola fragrans in vitro comparison with benzylaminopurine (BAP) kinetin (KT). Initiation was performed MS medium 0.5 mg/L BAP. For multiplication, MS, B5...

Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N6 –benzylaminopur...

A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of in vitro germinated seedling as primary explants on s...

Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7...

Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were o...

Objective: Here, we established the protocol for plant regeneration of Psoralea corylifolia via In-vitro micro propagation. Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine (BAP), Kinetin (Kn), 1-Naphthaleneacetic acid (NAA) and B5 vitam...

OBJECTIVE To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis (S. dulcis) L. METHODS Explants were inoculated on MS basal medium supplemented with kinetin and 6-benzylaminopurine for shoot bud induction. To enhance the shoot induction, various auxins like 3-indoleacetic acid or 3-indolebutyric acid or α-naphthylacetic acid were tested along with ...

Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benz...

Submitted: Apr 29, 2013; Accepted: May 30, 2013; Published: Jun 20, 2013 Abstract: This study was aimed to investigate the effects of growth regulators, including new one AN-16 (N-(3-phenylprop-2-yn-1-yl)-N-sodium butyldithiocarbamate, Patent 65377, Kazakhstan), on in vitro culture of some Vitis vinifera L. cultivars of Kazakhstan, European and Central Asia selections. Dormant buds of two culti...