DNA barcodes are short, unique ssDNA primers that "mark" individual biomolecules. To gain better understanding of biophysical parameters constraining primer-dimer formation between primers that incorporate barcode sequences, we have developed a capillary electrophoresis method that utilizes drag-tag-DNA conjugates to quantify dimerization risk between primer-barcode pairs. Results obtained with...

Topographic drag schemes depend on grid-scale representations of the average height, width, and orientation of the subgrid topography. Until now, these representations have been based on a combination of statistics and dimensional analysis. However, under certain physical assumptions, linear analysis provides the exact amplitude and orientation of the drag for arbitrary topography. The author p...

The blades of a vertical axis wind turbine (VAWT) rotor see an inconsistent angle of attack through its rotation. Consequently, VAWT blades generally use symmetrical aerofoils with a lower lift-to-drag ratio than cambered aerofoils tailored to maximise horizontal axis wind turbine rotor performance. This paper considers the feasibility of circulation controlled (CC) VAWT blades, using a tangent...

We review both present and past motions at major plate boundaries, which have the horizontal component in average 10 to 100 times faster (10–100 mm/yr) than the vertical component (0.01–1 mm/yr) in all geodynamic settings. The steady faster horizontal velocity of the lithosphere with respect to the upward or downward velocities at plate 5 boundaries supports dominating tangential forces acting ...

Scour is a phenomenon that occurs as a result of natural erosion by ground water flow and transport of seabed material. In this research, scour around the legs of marine structures is simulated using the FLOW3D.V.9.3™ commercial software. The general scour model consists of two components, namely, the lift and thrust forces. The drag force, that is inserted to the structure, is a combination of...

The hydrodynamics of a dispersed air-water system within a spouted column with a concentric draft tube and a conical base is simulated using CFD based on a two–fluid Euler–Euler (E-E) modeling framework and k-ε two-equation turbulence closures. The interaction between the dispersed gas phase and the continuous liquid phase is characterized by bubble–liquid interphase forces (drag, turbulent dis...

This article reports the active control of base flows using the experimental procedure. Active control of base pressure helps in reducing the base drag in aerodynamic devices having suddenly expanded flows. Active control in the form of microjets having 0.5 mm radius placed at forty-five degrees apart is employed to control the base pressure. The Mach numbers of the present analysis are 1.7, 2....

A computational study was conducted to better understand experimental results obtained from wind tunnel tests of a Mach 4 waverider model and a comparative reference configuration. The experimental results showed that the performance of the reference configuration was slightly better than that of the waverider model. These results contradict waverider design theory, which suggests that a waveri...