A high frequency shoot organogenesis and plant establishment protocol has been developed for Gerbera jamesonii from ex vitro leaf derived callus. The optimal callus was developed on Murashige and Skoog (MS) basal medium supplemented with 0.4 mg L 6-benzylaminopurine (BAP), 4.0 mg L -naphthalene acetic acid (NAA) and 3% (w/v) sucrose. Two callus types differing in their structures and growth rat...

Following in vitro infection with Agrobacterium rhizogenes wild strain (mannopine, 8196) and two A. tumefaciens transconjugant strains (C58C1 pArA4abc and C58C1 pArA4b) transformed (hairy) roots were induced in pumpkin (C. pepo L.) cotyledons. The presence of pRi T-DNA in pumpkin long-term hairy root cultures was determined by Southern hybridization. The influence of plant growth regulators on ...

High frequency plant regeneration in A. longifolia (L.) was achieved from leaf explant implanted on MS basal medium supplemented with NAA (0.5 mg/l) + BA (2.0 mg/l) through intervening callus phase. Well-developed shoots (>3cm) were successfully rooted on MS medium supplemented with NAA (0.1 mg/l). Protein and total soluble sugar contents were maximum during organogenesis and multiple shoot ind...

different grapevine cultures that were inoculated with Plasmopara viticola as a host pathogen and Pseudoperonospora cubensis as a non-host pathogen. The results of the expression analysis showed that the PR1 mRNA level in Vitis vinifera cv. Riesling and Vitis riparia cv. Gloire de Montpellier is mainly affected by the culture system. PR1 is constitutively expressed in callus cultures. In vitro ...

A study of callus induction of Tecomella undulata was conducted using a various concentrations of PGRs namely cytokinins such as BAP and KIN and auxins such as 2, 4-D, NAA and IAA. Internodal explants were aseptically cultured on MS medium supplemented with different concentrations (1.03.0 mg/l) of BAP, KIN, 2, 4-D, IAA and NAA. After two weeks callus was formed at the cut surface of the explan...

The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined...

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obta...

Embryogenic callus culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic embryogenesis In vitro cell suspension cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mi...