Bacteriorhodopsin, the only protein of the purple membranes of halobacteria [ 11, acts as a light-driven proton pump, translocating protons from the inner to the outer side of the plasma membrane [2]. The lightinduced asymmetric proton distribution contributes to the membrane potential by up to lo-40 mV [3]. Assuming a membrane thickness of 50 A, this potential difference corresponds to an elec...

Results are presented demonstrating that the backbone of the active site lysine of bacteriorhodopsin undergoes light-induced structural alterations during bacteriorhodopsin-mediated light-induced proton pumping. This conclusion is based on difference Fourier transform infrared spectroscopy of isotopically labeled bacteriorhodopsin. The data demonstrate that the backbone carbonyl of lysine achie...

The angular dependence of the hyper-Rayleigh scattered light intensity from a suspension of bacteriorhodopsin has been analyzed. The observation calls for combination of the second-order nonlinear hyperpolarizability of the retinal chromophore with the linear refractive index of the apo-protein matrix. The structuring of the small nonlinear chromophore in the large linear matrix imparts propert...

bacteriorhodopsin (br) is a retinal protein that is a light-driven proton pump and has an important role in photosynthesis in archaebacterium halobacterium salinarum. the br molecule absorbs light and photochemical changes occur in it, and different intermediates will be produced in its photochemical cycle that some of them like p and q intermediates have a long half-life. there have been many ...

Films of oriented bacteriorhodopsin have been formed in polyvinyl alcohol with excellent optical quality. Images with high contrast have been impressed and erased on these films. Second-harmonic microscopy has been used to read the image on a bacteriorhodopsin-polyvinyl alcohol film without erasure. The potential of these films for molecular information storage and computation is discussed.

today, using new and renewable energy sources to replace fossil fuels is greatly expanded. the solar cells are devices that convert solar energy to electrical energy. solar cells based on organic materials, especially proteins, are one of these solar cells. for example, bacteriorhodopsin (br) which is one of the membrane proteins in halobacterium salinarum could be used in solar cells and parti...

Molecular interactions and mechanical properties that contribute to the stability and function of proteins are complex and of fundamental importance. In this study, we used single-molecule dynamic force spectroscopy (DFS) to explore the interactions and the unfolding energy landscape of bovine rhodopsin and bacteriorhodopsin. An analysis of the experimental data enabled the extraction of parame...

The photochromic property of bacteriorhodopsin films is exploited in the application of a focusing schlieren optical system for the visualization of optical phase information. By encoding an image on the film with light of one wavelength and reading out with a different wavelength, the readout beam can effectively see the photographic negative of the original image. The potential advantage of t...