Multiple stains have been historically utilized in electron microscopy to provide proper contrast and superior image quality enabling the discovery of ultrastructures. However, use these microbiological viability assessment has limited. Phosphotungstic acid (PTA) staining is a common negative stain used scanning (SEM). Here, we investigate feasibility new SEM-PTA assay, aiming determine both vi...

background : verbascum thapsus (vt) is a medicinal plant that chemical constituents of it revealed the presence of biologically active compounds with antibacterial properties. the aim of the present study was to evaluate the antimicrobial and anti-adherent activities of ethanol extract of (vt) against of three oral streptococci in vitro. methods : in this study, biofilm formation of s. mutans 1...

Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...

BACKGROUND The aim of this study was to confirm recent results from a previous study focussing on the development of a method to measure the bacterial translocation through puncture holes in surgical gloves under real surgical conditions. METHODS An established method was applied to detect bacterial migration from the operating site through the punctured glove. Biogel double-gloving surgical ...

Aim: The present study aims at screening the phytochemical components and evaluates antioxidant, anti-bacterial, anti-inflammatory activity of fruit Piper schmidtii, an endemic plant species from Nilgiris, Tamil Nadu. Materials Method: different polar solvents such as petroleum ether, chloroform, ethyl acetate, methanol, water were used extraction was carried out using soxhlet apparatus. extrac...

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the s...