The objective of the present study was to determine the grades of nonspecific bacterial infection of genitalia of repeat breeding cattle by a simple and rapid test under field condition. For this purpose, a total of 100 crossbred Jersey cows comprising of 80 repeat breeding animals presented for treatment and 20 normal cyclic (control group) animals presented for artificial insemination at thei...

background bacterial meningitis is still considered as one of the most dangerous infectious diseases, which causes numerous complications and high mortality if not diagnosed and treated timely. objectives this study was performed to determine antimicrobial resistance patterns of bacterial pathogens isolated from acute bacterial meningitis at tabriz children educational-health care center in ira...

Rapid and accurate identification of mastitis pathogens is important for disease control. Bacterial culture and isolate identification is considered the gold-standard in mastitis diagnosis but is time-consuming and results in many culture-negative samples. Identification of mastitis pathogens by PCR has been proposed as a fast and sensitive alternative to bacterial culture. The results of bacte...

objective conjunctivitis is a common disease in neonates. several bacterial and viral infectious agents may cause it. tear circulation and blinking has protective effects from bacterial colonization in the conjuntival sac. reduced blinking increases the possibility of bacterial conjunctivitis. we studied the effect of eye patches on bacterial colonization of the conjunctival sac.  methods in 10...

PURPOSE To compare polymerase chain reaction (PCR) to microbial culture for the detection and identification of bacterial and fungal pathogens in microbial keratitis. DESIGN Prospective cohort study. METHODS A total of 108 consecutive corneal ulcers were cultured and analyzed by PCR using pan-bacterial and pan-fungal primers. PCR products were cloned, sequenced, and compared to culture resu...

staphylococcus spp., is a common bacteria in cattle mastitis. detection of the low number of bacteria requires a sensitive method. we used multiplex polymerase chain reaction (m-pcr) assay to detect the genes encoding sea, seb, sec toxins, that are specific in s. aureus bacteria. the gene of 23s rrna that is conserved in all staphylococcus spp., also was used. due to the stiffness and low effec...

Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA analyses were compared. A total of 168 chronic wounds were studied. The majority of bacteria iden...

  Background : An accurate and prompt diagnosis of bacterial arthritis is essential for earlier treatment and a good outcome. Superantigens produced by Staph. Aureus are among the most lethal toxins. The paper objective was Identification of common bacterial antigens and S.aureus superantigens in synovial fluid (SF) of children with negative culture and direct smear for other bacteria except fo...

REASONS FOR PERFORMING STUDY The influence of synovial fluid culture on short- and long-term prognosis of cases with septic synovitis requires study. HYPOTHESES Horses with a positive bacterial culture from septic synovial fluid are less likely to survive or return to successful athletic function than those with a negative bacterial culture from septic synovial fluid. METHODS Records of mat...