Influenza virus A (H1N1) is an important subtype of influenza virus that makes numerous consequences throughout the world. In the first step of viral attachment, main antigenic site of the HA1 domain from the globular head of hemagglutinin (HA) binds to human cell receptors, starting the disease process. In order to produce recombinant subunit protein vaccines, we focus on the nucleotide sequen...

BACKGROUND Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. Ectodomain of influenza M2 protein is a surface and highly conserved protein. M2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. OBJECTIVES In this study, a recombinant baculovirus containing M2e and cholera toxin subunit B fus...

Introduction: In recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. In this regard, producing a universal vaccine based on virus-like-particles (VLP) could be an ideal approach.  Methods: In this study, the full-length ORF of influenza hemagglutini...

odv-e25(e25) is one of the core genes of baculoviruses. To investigate how it functions in the replication cycle of a baculovirus, a number of Autographa californica multiple nucleopolyhedrovirus recombinants with e25 under control of the promoter of immediate early gene ie1, or the promoter of the very late hyperexpressed gene p10, were constructed using a bacmid system, and the effects of ear...

introduction: in recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. in this regard, producing a universal vaccine based on virus-like-particles (vlp) could be an ideal approach.  methods: in this study, the full-length orf of influenza hemagglutinin (ha...

results primary, secondary, and 3d structure of cchfv gn were obtained and pcr reaction with m13 forward and reverse primers confirmed the generation of recombinant bacmid dna harboring gn coding region under polyhedron promoter. conclusions characterization of the detailed structure of cchfv gn by bioinformatics software provides the basis for development of new experiments and construction of...

Here we describe a convenient method to generate homologous recombinant baculoviral genomes in E. coli. The recombination takes place with the aid of recombination enzymes provided by the phage lambda Red system between a bacmid (a baculoviral genome that can replicate in bacteria) and a linear fragment. Proof of concept was provided when the cathepsin gene (v-cath) of the Helicoverpa armigera ...

Genetically modified baculoviruses offer a promising alternative to chemical insecticides in the control of agricultural and forest insect pests. A novel bacmid, HaBacYH6, was constructed in which the ecdysteroid UDP-glucosyltransferase gene (egt) was replaced with a bacterial replication cassette containing a mini-F replicon, a kanamycin resistance gene, and the attTn7 site. Insertion of the e...

Repeated baculovirus infections in cultured insect cells lead to the generation of defective interfering viruses (DIs), which accumulate at the expense of the intact helper virus and compromise heterologous protein expression. In particular, Autographa californica multicapsid nucleopolyhedovirus (AcMNPV) DIs are enriched in an origin of viral DNA replication (ori) not associated with the homolo...

conclusions the results of the present study showed that the baculovirus expression system (sf9 cells) was able to express truncated orf2 and truncated orf2-nsp4 proteins as a potential candidate vaccine. background the hepatitis e virus (hev) accounts for hepatitis e infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. the baculovirus expression...