The particulate enzyme fraction from mung bean (Phaseolus aureus) seedlings catalyzes the incorporation of mannose from GDP-[(14)C]mannose into mannosyl-phosphoryl-dolichol and of N-acetylglucosamine from UDP-[(3)H]N-acetylglucosamine into N-acetylglucosamine-pyrophosphoryl-polyisoprenol. Bacitracin inhibits the transfer of both of these sugars into the lipid-linked saccharides with 50% inhibit...

The inhibition by bacitracin of the enzymatic dephosphorylation of C(55)-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C(55)-isoprenyl pyrophosphate form a complex...

Snoke, John E. (University of California, Los Angeles), and Neal Cornell. Protoplast lysis and inhibition of growth of Bacillus licheniformis by bacitracin. J. Bacteriol. 89:415-240. 1965.-The growth of Bacillus licheniformis is inhibited by bacitracin, the antibiotic which the organism itself produces. The effect of the antibiotic is confined to the early growth phase of the organism. Bacitrac...

Bacterial cultures of bacillus species capable of producing bacitracin were isolated from local habitats (garden soil, timber yard soil and sea water) and screened for the production of bacitracin on nutrient media against test organisms viz: Micrococcus luteus,(ATCC 9341) & Staphylococcus aureus (ATCC 13565).Optimization of culture conditions and media composition for bacitracin production was...

Bacitracin is a widely used metal-dependent peptide antibiotic produced by Bacillus subtilis and Bacillus licheniformis with a potent bactericidal activity directed primarily against Gram-positive organisms. This antibiotic requires a divalent metal ion such as Zn(II) for its biological activity, and has been reported to bind several other transition metal ions, including Co(II), Ni(II), and Cu...

Bacitracin synthetase is an enzyme complex [ 1,2] composed of three complementary fractions called A, B and C [3] (see fig.1). F’hosphopantetheine which is covalently bound to the fractions [2,3,.5] is probably involved in the biosynthesis of bacitracin. Similar to gramicidin S and tyrocidine [6] its mechanism of synthesis has been described as a thiotemplate mechanism [7]. In the present work ...

1. The influence of Lactobacillus acidophilus alone or in combination with zinc bacitracin on the performance of laying hens was monitored over a period of 4 months. 2. Lactobacillus acidophilus improved egg production, food conversion and reduced the cholesterol concentration in the eggs, but zinc bacitracin had no effect when administered alone. 3. In combination, bacitracin had an adverse ef...

Analysis of hydrolysates of highly purified spore coats revealed only small quantities of ornithine, a component of bacitracin. We conclude that the peptide, bacitracin, is not a significant component of spore coats.

Hancock, R. (Harvard Medical School, Boston, Mass.), and P. C. Fitz-James. Some differences in the action of penicillin, bacitracin, and vancomycin on Bacillus megaterium. J. Bacteriol. 87:1044-1050. 1964.-Penicillin and cycloserine do not inhibit the growth of protoplasts of Bacillus megaterium, indicating that inhibition of cell-wall synthesis is the only significant process by which they inh...

Growth of Bacillus licheniformis in a chemically defined medium containing glucose and ammonium chloride yielded a doubling time of 1.00 h. Examination of the culture during exponential growth revealed a lack of heat-resistant spores together with a complete absence of detectable concentrations of bacitracin or extracellular serine protease. Replacement of glucose as the sole carbon source by g...