OBJECTIVE In-vitro red blood cell (RBC) partitioning of doxycycline was studied to determine whether doxycycline penetrates RBC and its concentration was assayed keeping in view its high lipophilicity. MATERIALS AND METHODS Standardization of doxycycline was performed in whole blood and plasma of cattle by microbiological assay using Bacillus subtillis ATCC 6633 as indicator organizm. Actual ...

Reaction of the amino acid D-phenylalanine ethyl ester (4) with cinnamaldehyde gave chiral Schiff base 5, which underwent an asymmetric Staudinger [2+2] cycloaddition reaction with phthalimidoacetyl chloride to give the monocyclic beta-lactam 6 as a single stereoisomer. Ozonolysis of 6 followed by reduction with lithium aluminum tri(tert-butoxy) hydride afforded the hydroxymethyl beta-lactam 8....

A novel 4-[(8-hydroxyquinolin-5-yl)methyl]aminobenzenesulfonamide (HQMABS) was synthesized by optimized reaction of 4-aminobenzenesulfonamide with 5-chloromethyl-8-hydroxyquinoline hydrochloride (CMHQ). Various oxinates of HQMABS were also prepared using Mn(II), Fe(II), Co(II), Ni(II), Cu(II), and Zn(II) metal salts. All compounds were analyzed by physicochemical, thermogravimetric and spectros...

The chemical and biological profiling of the methanolic extract floral part Costus lucanisianus have been carried out. was collected from Ogbia Local Government Area Bayelsa State. air dried, grinded extracted at room temperature in methanol. methanol mixture then concentrated under pressure. extract, MCL subjected to quantitative phytochemical screening, antioxidant antimicrobial profiling. re...

Background: Bacillus subtillis bacteria can cause meningitis, endocarditis, and eye infections, while Enterococcus meningitis in neonates endocarditis adults. Therefore, it is important to use hand sanitiser avoid bacterial infections. Eucalyptus an essential oil-producing plant that inhibit growth kill with its compounds, namely linalool pinocarveol. Purpose: To make a good eucalyptus oil test...

DNA supercoiling is a major regulator of transcription in all organisms. This process is regulated by type I and type II DNA topoisomerases that are targets for microbial antibiotics and/or anti-tumour drugs. Despite extensive studies in this field, no information is available on the response of Bacillus (B) species to eukaryotic DNA topoisomerase inhibitors. Here we found that B. thuringiensis...