Objective: Methotrexate (MTX) is a commonly used chemotherapeutic agent in clinical oncology practice and treatment of autoimmune diseases. The major side effect during mucositis. Recently published studies reported that this may be due to direct intestinal epithelial injury disturbance microbiota. In study, we aimed characterize the histological changes caused by MTX investigate effects safety...

Riboswitches are structured RNAs typically located in the 5' untranslated regions of bacterial mRNAs that bind metabolites and control gene expression. Most riboswitches sense one metabolite and function as simple genetic switches. However, we found that the 5' region of the Bacillus clausii metE messenger RNA includes two riboswitches that respond to S-adenosylmethionine and coenzyme B12. This...

Two types of white wheat bread (high- and low-type loaves) were investigated for rope spoilage. Thirty of the 56 breads tested developed rope spoilage within 5 days; the high-type loaves were affected by rope spoilage more than the low-type loaves. Sixty-one Bacillus strains were isolated from ropy breads and were characterized on the basis of their phenotypic and genotypic traits. All of the i...

Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of...

Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75...

Bağırsak mikrobiyotasındaki bir dengesizlik, kedilerdeki kronik enteropatide olduğu gibi gastrointestinal sistemdeki inflamasyon oluşumu için hazırlayıcı faktör olarak hizmet ettiği tespit edilmiştir. Probiyotik uygulamasının rahatsızlıkları azaltabileceği varsayımı göz önüne alındığında, ülkemizde enteropatili kedilerde rektal yoldan etkileri hiç araştırılmamıştır. Bu nedenle bu çalışmanın ama...