Forty-seven strains representing 14 different Bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (IMF) and subsequently assessed for adherence to, invasion of, and cytotoxicity toward HEp-2 and Caco-2 cells. Cell-free supernatant fluids from 38 strains (81%) were shown to be cytotoxic, 43 strains (91%) adhered to the test cell lines, and 23...

Quantitative real-time PCR (qRT-PCR) offers an alternative method for the detection of bacterial contamination in food. This method provides the quantitation and determination of the number of gene copies. In our study, we established an RT-PCR assay using the LightCycler system to detect and quantify the Bacillus cereus group species, which includes B. cereus, B. anthracis, B. thuringiensis, B...

Bacillus cereus infections The importance of Bacillus organisms, particularly B. cereus, in local infections has been largely overlooked because they are commonly encountered as contaminants in specimens or cultures. Turnbull et al. (J Clin Pathol 1979 ;32 :289-93) reported severe infections with B. cereus and prompted me to review the recent isolates in this laboratory. In the nine months, Nov...

The Bacillus cereus group bacteriophage TsarBomba, a double-stranded DNA Myoviridae, was isolated from soil collected in Saratov, Russia. TsarBomba was found to be similar to Bacillus phages BCP78 and BCU4, and to have a wide host range among Bacillus cereus group species.

New 1,3,4-thiadiazole, 5a-e, and 1,2,4-triazolecompounds 6a-c, containing a D,L-methionine moiety were synthesized by intramolecular cyclization of 1,4-disubstituted thiosemicarbazides 4a-e in acid and alkaline media, respectively. The potential antimicrobial effects of the synthesized compounds were investigated using the Staphylococcus aureus ATCC 25923, Bacillus antracis ATCC 8705, Bacillus ...

Article history: Received on: 15/03/2016 Revised on: 20/04/2016 Accepted on: 30/05/2016 Available online: 28/07/2016 The aim of the present research work was to optimize cholesterol oxidase production and partial sequencing of 16S rRNA gene region of Bacillus cereus strain KAVK4 by PCR. Bacillus cereus is a gram positive bacterium isolated from butter, Tamil Nadu, India and was cultured in a nu...

C1 is an endospore forming gram-positive rod isolated from snow-covered soil in Massachusetts. Colonies were white and feathery in appearance and grew after 22 hours. The isolate was motile with ellipsoidal spores that are subterminal. The strain grew best at temperatures between 5-25 ̊ C with a pH range between 5–7 and NaCl concentration 1% (w/v). Phylogenetic analysis based on 16S rRNA gene se...

Bacillus species (Bacillus cereus, Bacillus clausii, Bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. Three B. cereus strains were shown to persist in the mouse gastrointestinal tract fo...

Among 200 selected dairy samples 50 each of pasteurized milk, cream, butter and paneer were analyzed for the presence of Bacillus cereus the paneer samples were found to have the highest incidence of Bacillus cereus (52.94%) followed by cream sample (29.41%) and pasteurized milk sample (17.64%) however none of the butter samples were found to be contaminated with Bacillus cereus. These isolates...

The bacterial strain Bacillus cereus is closely related to Bacillus thuringiensis, although any genetic relationship between the two strains is still in debate. Using rep-PCR genomic fingerprinting, we established the genetic relationships between Brazilian sympatric populations of B. cereus and B. thuringiensis simultaneously collected from two geographically separate sites. We observed the fo...