We have engineered Escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the C₁₁ to C₁₅ (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we report here). We describe specific improvements that resulted in a 700-fold enha...

The Streptomyces glaucescens fabH gene, encoding beta-ketoacyl-acyl carrier protein (beta-ketoacyl-ACP) synthase (KAS) III (FabH), was overexpressed in Escherichia coli, and the resulting gene product was purified to homogeneity by metal chelate chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the purified protein revealed an Mr of 37,000, while gel filtrati...

Escherichia coli hosts expressing fabG of Pseudomonas aeruginosa showed 3-ketoacyl coenzyme A (CoA) reductase activity toward R-3-hydroxyoctanoyl-CoA. Furthermore, E. coli recombinants carrying the poly-3-hydroxyalkanoate (PHA) polymerase-encoding gene phaC in addition to fabG accumulated medium-chain-length PHAs (mcl-PHAs) from alkanoates. When E. coli fadB or fadA mutants, which are deficient...

The β-ketoacyl CoA synthase encoded by the KCS genes is a rate-limiting enzyme for synthesis of very-long-chain fatty acid (VLCFA), which catalyzes VLCFA elongation. Yellow horn (Xanthoceras sorbifolium) horticultural tree species known its kernel oil, has strong resistance to drought, cold, high temperature, and saline-alkali. conserved domain FAE1-CUT1-RPPA ACP-syn-III_C gene family were used...

Abstract Microalgal lipids and fatty acids are important components for achieving biofuel because of their potential high productivity. Although that have a hydroxy group adjacent to the end acyl chain might be an chemical feedstock, most algae do not accumulate it. To produce (ω−1)-hydroxy from 3-hydroxybutylyl-CoA, intermediate polyhydroxybutylate biosynthesis in cyanobacterium Synechocystis ...

A 30-kD coenzyme A (CoA)-binding protein was isolated from spinach (Spinacea oleracea) chloroplast soluble extracts using affinity chromatography under conditions in which 95% of the total protein was excluded. The 30-kD protein contains an eight-amino-acid sequence, DVRLYYGA, that is identical to a region in a 36-kD protein of unknown function that is encoded by a kiwifruit (Actinidia delicios...

Although the management of ischemic heart disease and chronic heart failure (CHF) has made considerable progress over the past years, CHF is still a tremendous medical burden. The metabolic and therapeutic approach might play a significant role in reducing disease progression and mortality rate. The metabolic modulator trimetazidine, a partial inhibitor of long chain 3-ketoacyl CoA thiolase act...

Roegneria kamoji Ohwi (Poaceae), a wild relative plant of wheat which is widely distributed across China, has become dominant and problematic weed in fields some regions. We have previously confirmed that R. highly tolerant to foliar-applied acetyl-CoA carboxylase (ACCase) acetolactate synthase (ALS) inhibitors (Triticum aestivum L.). The sensitivity pre-emergence (PRE) herbicides the basis fen...

The fatty acid elongase [often designated FAE or beta-(or 3-) ketoacyl-CoA synthase] is a condensing enzyme and is the first component of the elongation complex involved in synthesis of erucic acid (22:1) in seeds of garden nasturtium (Tropaeolum majus). Using a degenerate primers approach, a cDNA of a putative embryo FAE was obtained showing high homology to known plant elongases. This cDNA co...

Insects do not synthesize cholesterol; the 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) produced by HMG-CoA synthase is transformed to mevalonate by HMG-CoA reductase for the production of non-sterol isoprenoids, which are essential for growth and differentiation. To understand the regulation and developmental role of HMG-CoA synthase, we have cloned a 1658 bp cDNA that encompasses the entir...