Both CCL20 and human β-defensin 2 (hBD2) interact with the same membrane receptor and display chemotactic and antimicrobial activities. They are produced by airway epithelia in response to infectious agents and proinflammatory cytokines. Whereas Brucella spp. can infect humans through inhalation, their ability to induce CCL20 and hBD2 in lung cells is unknown. Here we show that B. abortus induc...

Smooth lipopolysaccharides (SLPS) from Brucella abortus contain A-epitopes against which the majority of serum antibodies are directed during infections. SLPS from Yersinia enterocolitica 0:9 possesses identical epitopes, which are the cause for serological cross-reactivity. All Brucella spp. possess M- and C-epitopes which are not present in Y. enterocolitica 0:9. In order to examine the usefu...

Th1 immune responses, characterized by production of gamma interferon (IFN-gamma), are associated with protective immunity to viruses and intracellular bacteria. Heat-killed Brucella abortus promotes secretion of Th1-inducing cytokines such as interleukin-12 (IL-12) and IFN-gamma and has been used as a carrier to induce Th1 responses to vaccines. To explore which bacterial constituents could me...

A rapid and simple method for the real time monitoring of Brucella abortus CSP-31 (B. abortus CSP-31) by using Surface Plasmon resonance (SPR) is first time reported. SPR and electrochemical impedance spectroscopy (EIS) were used to characterize the B. abortus CSP-31 interaction on carboxymethyldextran modified gold disc with antibody. The EIS data revealed a decrease in impedance after interac...

BACKGROUND Brucella abortus can proliferate within professional and nonprofessional phagocytic host cells and thereby successfully bypass the bacteriocidal effects of phagocytes. However, the intracellular survival mechanism and factors of virulence are not fully understood. METHODS We have investigated the role of the regulator of G protein signaling 2 (RGS2), an intracellular calcium ([Ca(2...

Baker, Phillip J. (University of Wisconsin, Madison), and J. B. Wilson. Chemical composition and biological properties of the endotoxin of Brucella abortus. J. Bacteriol. 90:895-902. 1965.-The ability of endotoxin to induce hypoferremia in mice was used to measure the specific activity of various endotoxin preparations of Escherichia coli and Brucella abortus and to determine the endotoxin cont...

The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study, B. abortus Omp28, a group 3 antigen, was amplified by PCR and cloned into a maltose fusion protein expression system. Recombinant Omp28 (rOmp28) was expressed in Escherichia coli and was t...

Brucella contaminates people with raw milk, raw dairy products and especially with cheese made from raw milk. This study has aimed to detect Brucella abortus and Brucella melitensis in fresh cheese (Coban Cheese, 100-mold), traditional Afyon Tulum Cheese (Aged Skin Bag Cheese) (50-mold) and sheep-lamb tulum (50-mold) samples provided from Afyonkarahisar province. Conventional Culture Method has...

The Brucella abortus type IV secretion system (T4SS), encoded by the virB operon, is essential for establishing persistent infection in the murine reticuloendothelial system. To gain insight into the in vivo interactions mediated by the T4SS, we compared host responses elicited by B. abortus with those of an isogenic mutant in the virB operon. Mice infected with the B. abortus virB mutant elici...

background: adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. despite immunostimulatory properties, the use of bacterial lipopolysaccharide (lps) as an adjuvant has been hampered due to its toxicity and pyrogenicity. brucella abortus lps is less toxic and has no pyrogenic properties compared to lps from other gram negat...