Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...

objectives the aim of this was to indentify the presence of a/h9n2 virus among different high risk occupational groups, in tehran and qazvin provinces in seasonal outbreak in iran. background in the last decade h9n2 avian influenza viruses had caused outbreaks in poultry in many parts of the world. this subtype could infect other animals such as human and pig. avian h9n2 virus has acquired rece...

salmonella enterica is considered as one of the major pathogens in public health, worldwide. diseases caused by salmonella enterica serovars are especially prevalent in developing countries. it is one of the emerging pathogen in food-borne diseases, which is often found in contaminated chicken eggs. the aim of this study was to apply a pcr–rflp method using sau3ai and hhai restriction endonucle...

background: avian pathogenic escherichia coli (apec), which is the causative agent of colibacillosis, harbors several putative virulence genes. an important trait of apec for both poultry and public health is antibiotic resistance. objectives: in the present study, some potential virulence genes of apecs isolated from northeast of iran and their resistance to the quinolones antibiotics were stu...

an adult native cock (gallus gallus domesticus) referred to the aviary clinic with multiple different sizes of round dermal nodules. the bird died few days later, and was then submitted for further evaluation. macroscopic and microscopic examinations as well as a pcr test were done to identify type and cause of the tumor. in histopathological assessment of biopsy specimen, it consisted of inter...

Influenza is a highly contagious zoonotic disease in the world. Avian H9N2 influenza virus is a significant pandemic pathogen widely distributed throughout the world. Pet ownership has been documented as a risk factor for infection transmission to human. Considering major public health concern, the prevalence of antibodies against avian H9N2 influenza virus was evaluated in 170 serum samples of...

capsules from a range of pathogenic bacteria are the key determinants of virulency. the capsule hasbeen implicated in virulence of pasteurella multocida. in this study a type-specific polymerase chain reaction(pcr) assay was used for capsular typing of 39 avian p. multocida isolates from iran. the pcr amplified afragment of 1044 bp from all of tested isolates. it was found that all avian p. mul...

In early 2007, H2N3 influenza virus was isolated from a duck and a chicken in two separate poultry flocks in Ohio. Since the same subtype influenza virus with hemagglutinin (H) and neuraminidase (N) genes of avian lineage was also identified in a swine herd in Missouri in 2006, the objective of this study was to characterize and compare the genetic, antigenic, and biologic properties of the avi...

Most highly pathogenic avian influenza viruses (AIV) emerge after field passage of non-pathogenic AIVs in birds. The outbreak of low-pathogenic H7N1 avian influenza virus in Italy during 1999-2000 followed by outbreak of highly pathogenic H7N1 avian influenza virus is one example in this regard. This experiment has been designed to investigate the effect of pre-infection of birds with LPAI on s...