نتایج جستجو برای: automated sequencer
تعداد نتایج: 130519 فیلتر نتایج به سال:
The Scottish Meningococcus and Pneumococcus Reference Laboratory provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. The main tests used for the laboratory confirmation of meningococcal disease are culture, the polymerase chain reaction (PCR), antibody testing, and more recently DNA sequencing. This paper describes the automation of...
An automated ultra-sensitive single-lane DNA sequencer based on single photon counting fluorescent detection technique has been developed and tested. The instrument is intended for individual researchers who carry out a limited number of sequencing experiments (about 10 bp/year, the size typical for small-scale research projects). Due to the use of single photon detection, sensitivity of the in...
Human factor D purified to homogeneity by a modified procedure was subjected to NH2-terminal amino acid sequence analysis by using a modified automated Beckman sequencer. We identified 48 of the first 57 NH2-terminal amino acids in a single sequencer run, using microgram quantities of factor D. The deduced amino acid sequence represents approximately 25% of the primary structure of factor D. Th...
The demand for high-throughput DNA profiling has increased with the introduction of national DNA databases and has led to the development of automated methods of short tandem repeat (STR) profile production; however, a potential bottleneck still exists at the gel electrophoresis stage. Capillary electrophoresis sequencers capable of processing 96 samples with considerably reduced manual interve...
Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analysis of footprint patterns on a standard automated laser fluorescent (A.L.F.) DNA Sequencer. The co...
Confirmation of mutations mostly involves DNA sequencing. The conventional manual radioactive labeling sequencing protocol may be conceived as the method of choice because of the perception that the capital and operational costs of automated sequencers are high. Velickovic et al. (3) reduced the cost of DNA sequencing by running 48 or 64 samples on a 36-lane model 377-01 ABI PRISM automated se...
Maturity-onset diabetes of the young (MODY) is a form of NIDDM characterized by an early age of onset and autosomal-dominant inheritance (1). So far, three MODY-causing genes have been identified; namely, the hepatocyte nuclear factor4a (HNF-4a)/M0DYl on chromosome 20q, the glucokinase/M0DY2 on chromosome 7p, and the HNFla/M0DY3 on chromosome 12 q (2-4). The identification of the MODY genes has...
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