Ascorbate peroxidase, a haem protein (EC 1.11.1.11), efficiently scavenges hydrogen peroxide (H(2)O(2)) in cytosol and chloroplasts of plants. In this study, a full-length coding sequence of thylakoid-bound ascorbate peroxidase cDNA (TatAPX) was cloned from a drought tolerant wheat cultivar C306. Homology modeling of the TatAPX protein was performed by using the template crystal structure of ch...

potato (solanum tuberosum l.) auxiliary buds c.v. white desiree were cultured in ms medium containing 0, 50, 100, 150 and 200 μm concentrations of silver thiosulfate (sts) under in vitro condition. after eight weeks, the effect of silver ions (ag+) in the form of silver thiosulfate complex (sts), as an ethylene action inhibitor, on chlorophyll contents of leaves, ascorbate peroxidase, guaiacol ...

A proportion of the plant's l-ascorbate (vitamin C) occurs in the apoplast, where it and its metabolites may act as pro-oxidants and anti-oxidants. One ascorbate metabolite is 2,3-diketogulonate (DKG), preparations of which can non-enzymically generate H2O2 and delay peroxidase action on aromatic substrates. As DKG itself generates several by-products, we characterised these and their ability t...

This study examines phenolic peroxidase (POX) in Rhizophora mangle L. leaves in order to assess its role in phenolic manipulation and H 2 O 2 scavenging. Sun-exposed and understorey leaves experiencing varying degrees of nutrient stress were analysed from an oligotrophic cay off the coast of Belize. POX activity was unaffected by growth environment, but increased throughout leaf development and...

Induction of zeaxanthin formation and the associated nonphotochemical quenching in iodoacetamide-treated, non-CO(2)-fixing intact chloroplasts of Lactuca sativa L. cv Romaine is reported. The electron transport needed to generate the required DeltapH for zeaxanthin formation and nonphotochemical quenching are ascribed to the Mehler-ascorbate peroxidase reaction. KCN, an inhibitor of ascorbate p...

This data article is referred to the research article entitled The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration by Uarrota et al. (2015). Food Chemistry 197, Part A, 737-746. The stress duo to PPD of cassava roots leads to the formation of ROS which are extremely harmful and accel...

Hydrogen peroxide (H(2)O(2)) scavenging systems of spruce (Picea abies) needles were investigated in both extracts obtained from the extracellular space and extracts of total needles. As assessed by the lack of activity of symplastic marker enzymes, the extracellular washing fluid was free from intracellular contaminations. In the extracellular washing fluid ascorbate, glutathione, cysteine, an...

All aerobic biological systems, including N(2)-fixing root nodules, are subject to O(2) toxicity that results from the formation of reactive intermediates such as H(2)O(2) and free radicals of O(2). H(2)O(2) may be removed from root nodules in a series of enzymic reactions involving ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. We confirm here the presence of thes...

In order to understand the response of tobacco to salt stress, antioxidant enzyme activities, plant biomass and ion content were analyzed in two oriental tobacco genotypes (Basma 31 and SPT 406). Tobacco plants were exposed to 0, 50, 100, 150 and 200 mM NaCl for 12 days. The fresh and dry weight as well as shoot and root length of Basma 31 were greater than those of SPT 406 under increasing sal...

Deficiency of nitrogen may lead to decrease in yield and yield potential of crop plants. An experiment was done under glasshouse conditions in order to investigate nitrogen levels and salicylic acid interaction on some physiologic traits of borage (Borago officinials L.). In this experiment various levels of nitrogen (including; 27.5, 55, 110, 220 and 330 mg/L from ammonium nitrate) and salicyl...