PURPOSE Cytological examination of ascitic fluid is critical for clinical management of patients with peritoneal or pelvic diseases. Such morphological examination can only achieve a sensitivity of <62%, and thus a molecular test that is able to distinguish benign versus malignant ascites could be clinically useful. In this study we determined the presence of allelic imbalance (AI) in tumor-rel...

OBJECTIVE The study was designed to elucidate the correlation of spontaneous bacterial peritonitis (SBP) with aetiology of liver cirrhosis, overall mortality, ascitic fluid and systemic microbial infections. METHODS Sixty three patients with cirrhosis of the liver were included in this study. These patients were diagnosed on the basis of clinical evaluation, biochemical investigation, ultraso...

The macrophage-like cell line Mm-i consists of spontane ously differentiated cells of a clonal line of mouse myeloid leukemia cells (Ml). Mm-i cells were seeded in soft agar on a harder agar base in the presence or absence of ascitic fluid from rats bearing hepatoma AH-i 30. Although a few colonies developed in the absence of ascitic fluid, the number of cob nies was significantly increased by ...

BACKGROUND The classification of ascites as 'exudative' and 'transudative' based on ascitic fluid total protein (AFTP) has been challenged in many clinical conditions like cardiac ascites, patients on prolonged diuretic therapy and malignant ascites because it had poor diagnostic efficacy. These drawbacks have led to the development of another approach to classify ascites, which is based on Ser...

BACKGROUND/AIMS The diagnosis of spontaneous bacterial peritonitis (SBP) is based on a polymorphonuclear leukocytes (PMNs) exceeding 250/μL in ascitic fluid. The aim of the study was to evaluate serum procalcitonin and ascitic fluid calprotectin as accurate diagnostic markers for detecting SBP. METHODS Seventy-nine patients with cirrhotic ascites were included. They were divided into a SBP gr...

Spontaneous bacterial peritonitis is defined by a positive ascitic fluid bacterial culture and an elevated ascitic fluid absolute polymorphonuclear count (≥250 cells/mm3) without an evident intra-abdominal, surgically treatable source of infection. Transient ascites is well documented in patients with extrahepatic portal venous obstruction but spontaneous bacterial peritonitis complicating extr...

The age of in vivocultures of ascites tumor cells was shown to determine in part the rate of labeling of phosphorus compartments of the cells (nucleic acids, phospholipides, and phosphoprotein) with P32 (7). During the course of this experimentation, the question arose whether this effect of age upon radioactive labeling resided in the cell itself or in the ascitic fluid bathing the cells in vi...

Spontaneous bacterial peritonitis (SBP) is a frequent and severe complication in cirrhotic patients with ascites. Although identifying the pathogen(s) plays a major role in the management of infectious diseases, ascitic fluid cultures often show negative results in patients with clinical signs and symptoms of SBP, and ascitic fluid cell analyses are the gold standard method for diagnosing SBP. ...

OBJECTIVE To evaluate sensitivity of effusion cytology in detecting malignancy. MATERIALS AND METHODS Effusion cytology was studied from 37 malignancy associated and 28 non malignancy associated ascitic fluid samples. RESULTS Out of 65 cases, 44 (67.7%) effusions were reported negative, 15 (23.1%) were positive and 6 (9.2%) were suspicious for malignancy. Thus total 21 effusions (32.3%) wer...

BACKGROUND & OBJECTIVE CA-125, an ovarian tumor marker is known to increase in non malignant conditions such as tubercular and non tubercular pleuritis and ascites. We undertook this study to evaluate non-specific rise in CA-125 levels in conditions associated with pleural effusion and ascites and also to understand the mechanism of its secretion. METHODS CA-125 levels in 38 pleural and 46 as...