Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys(59)/Cys(64) adjacent to the flavin ring of FAD and another center consisting of Cys(497)/selenocysteine (SeCys)(498) near the C terminus. We now show that the C-terminal Cys(497)-SH/SeCys(498)-Se(-) of NADPH-reduced enzyme, after anaerobic dialysis, was converted to a thioselenide on incubation with excess ox...

Epidemiology and Transmission 487 Staphylococcus aureus 487 Coagulase-Negative Staphylococci 488 Microbiology 489 Staphylococcus aureus 489 Coagulase-Negative Staphylococci 490 Pathogenesis of Disease 490 Virulence Factors of Staphylococcus aureus 490 Epithelial Attachment and Invasion 490 Innate Immune Resistance 491 Secreted Toxins 492 Quorum Sensing and Regulation of Virulence Factor Express...

Obesity is a pandemic and major risk factor for cancers. The reduction of obesity would have been an effective strategy for cancer prevention, but the reality is that worldwide obesity has kept increasing for decades, remaining a major avoidable cancer risk secondary only to smoke. The present studies suggest that vitamin D may be an effective agent to reduce obesity-associated cancer risks in ...

Nitric oxide (NO) plays a pivotal role in tumorigenesis, particularly with relation to cancer cell invasion and metastasis. NO can reversibly couple to cysteine thiols to form an S-nitrosothiol, which regulates the enzymatic activities of target proteins. c-Src is a tyrosine kinase that promotes cancer cell invasion and metastasis. Interestingly, c-Src can be activated by NO stimulation. Howeve...

Emerging evidence suggests that BRCA1 pathway contributes to the behavior of sporadic triple negative breast cancer (TNBC), but little is known about the mechanisms underlying this association. Considering the central role that microRNAs (miRNAs) play in gene expression regulation, the aim of this study was to identify miRNAs specifically deregulated in TNBC and investigate their involvement in...

ability [114]. abnormality [212]. abnormally [415]. Absence [410]. abstract [303]. abundance [498]. across [498]. actin [382, 297, 313, 279, 159]. acting [406]. action [301, 241]. activate [306]. activating [79]. activation [379, 486, 276]. active [20]. activity [44, 226, 264]. actomyosin [261, 373]. actomyosin-S1 [373]. acute [201]. Adam [471]. Adaptation [543, 326, 485, 155, 506, 398]. adapti...

Many virus infections give rise to surprisingly limited T-cell responses directed to very few immunodominant determinants. We have been examining the cytotoxic T-lymphocyte (CTL) response to herpes simplex virus type 1 (HSV-1) infection. Previous studies have identified the glycoprotein B-derived peptide from residues 498 to 505 (gB(498-505)) as one of at least three determinants recognized by ...