We describe the development and application of a sensitive high-resolution fluorescence alkaline phosphatase (APase)-Fast Red immunocytochemical (ICC) staining method in combination with fluorescence in situ hybridization (ISH) and bromodeoxyuridine (BrdU) detection. The high fluorescence intensity, accurate localization, and advantageous slow-fading properties make the APase-Fast Red reaction ...

Soybean [Glycine max (L.) Merr.] contains two proteins called vegetative storage proteins (VSPs) that function as temporary storage reserves, but are also closely related to plant acid phosphatases of the haloacid dehalogenase (HAD) superfamily. This study examined the biochemical basis for the relatively low catalytic activity previously reported for these VSPs. The specific activity of purifi...

The high phosphorus (P) acquisition ability of crops can reduce their dependence on artificial inorganic phosphate (Pi) supplementation under Pi-limited conditions. Melon (Cucumis melo L.) is vulnerable to Pi deficiency. This study was carried out explore the morphological and physiological responses melon low-Pi stress a hydroponic system. results show that significantly disturbed nutrient hom...

phosphorus (p), in the form of phosphate ion (pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. the objective of the present study was to assess the influence of fungal infection on pi metabolism in compare to the effects of phosphate stress in arabidopsis. quantification of total p contents showe...

Optimal conditions for maximal extractions of alkaline phosphatase (APase) present in the thymuses, spleens, livers, and kidneys of C57BL/6, SJL/J, and AKR strains of mice, leukemic and nonleukemic, were selected. Standard assay conditions for measuring APase activities were found by studying the dependencies of the observed enzymatic activities on pH, concentrations of MgCl2, NaCl, and Tris. M...

introduction: bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. material and methods: the stromal cells of bone marrow obtained from 4 to 6 weeks old spruge-dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. the cells were cultured in either dmem medium containing 15% fetal c...

Induction of secreted acid phosphatase (APase) is a universal response of higher plants to phosphate (Pi) limitation. These enzymes are thought to scavenge Pi from organophosphate compounds in the rhizosphere and thus to increase Pi availability to plants when Pi is deficient. The tight association of secreted APase with the root surface may make plants more efficient in the utilization of soil...

Harmful effects of contaminants on the ecosystem and humans cannot be assessed by standard chemical analyses of environmental samples, therefore toxicity tests using live organisms or cells represent a vital part of environmental monitoring. Biological methods based on microorganisms as test-species, have already successfully been applied to environmental toxicity/genotoxicity assessment. The e...

PURPOSE To develop a high-resolution histologic technique to study the postmortem human choroidal vasculature in dual perspective: vascular pattern in the flat perspective and structure in cross sections. METHODS Fresh whole human choroids were denuded of retinal pigment epithelium, fixed, incubated for enzyme histochemical demonstration of alkaline phosphatase (APase) activity, bleached with...

This work studied the acid phosphatase (APase) activity from culture medium (extracellular, eAPase) and mycelial extract (intracellular, iAPase) of Aspergillus niger F111. The influence of fungus growth and phosphate concentration of the media on the synthesis and secretion of phosphatase was demonstrated. The effects of pH, substrate concentration and inorganic and organic compounds added to t...