A self-organizing feature map (SOM) was used to represent vehicle-following and to analyze the heterogeneities in vehicle-following behavior. The SOM was constructed in such a way that the prototype vectors represented vehicle-following stimuli (the follower's velocity, relative velocity, and gap) while the output signals represented the response (the follower's acceleration). Vehicle trajector...

Current research on antigen specific CD4+ T cells indicates that there is functional and phenotypic heterogeneity within these populations, but the extent of this heterogeneity is poorly described. The CD134/CD25 assay allows live isolation of antigen specific cells in vitro for down-stream molecular analysis. Antigen specific CD4+ T cells were examined at the molecular level by lineage specifi...

Abstract Atypical B cells are generated during a normal adaptive immune response, yet the role of these in protective immunity is still largely unknown. Here, we used chronic Plasmodium falciparum exposure as model to gain insight into function and potential heterogeneity atypical response infection. To this end, performed single-cell sequencing on circulating from malaria-experienced individua...

PURPOSE Metastasis heterogeneity presents a significant obstacle to the development of targeted cancer therapeutics. In this study, we sought to establish from a large series of human melanoma metastases whether there exists a determined pattern in tumor cellular heterogeneity that may guide the development of future targeted immunotherapies. EXPERIMENTAL DESIGN From a cohort of 1,514 patient...

Purpose: Metastasis heterogeneity presents a significant obstacle to the development of targeted cancer therapeutics. In this study, we sought to establish from a large series of human melanoma metastases whether there exists a determinedpattern in tumor cellular heterogeneity thatmay guide the development of future targeted immunotherapies. Experimental Design: From a cohort of 1,514 patients ...

Heterogeneity with regard to affinity of anti-hapten antibody was demonstrated at the cellular level in mice. The heterogeneity was shown at the level of single antibody-forming cells using hapten inhibition of hemolytic antibody plaque formation as a measure of affinity. The affinity increased with time after immunization. A high antigen dose initially resulted in relatively low affinity antib...