In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-seroty...

A study was conducted with the RiboPrinter, an automated ribotyping system, to evaluate its ability to identify and characterize isolates of Salmonella from broiler operations. Isolates of Salmonella obtained from a local broiler company were serotyped by a reference laboratory and ribotyped using the RiboPrinter. The RiboPrinter generated ribotype patterns by probing EcoRI digests of Salmonell...

Clostridioides difficile is a Gram positive spore-forming rod and mainly responsible for nosocomial diarrhea in developed nations. Molecular antimicrobial surveillance important monitoring the strain composition including genotypes of high epidemiological importance such as ribotype 027 (RT027) corresponding resistance patterns. 1535 isolates obtained from samples sent between 2014 2019 to Germ...

Microbial source tracking (MST) uses various approaches to classify fecal-indicator microorganisms to source hosts. Reproducibility, accuracy, and robustness of seven phenotypic and genotypic MST protocols were evaluated by use of Escherichia coli from an eight-host library of known-source isolates and a separate, blinded challenge library. In reproducibility tests, measuring each protocol's ab...

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein analysis, automated ribotyping, and phenotypic tests (e.g., cell morphology, gas production from glucose, growth and acid production on homofermemtative-heterofermentative differential (HHD) agar medium, sugar fermentation patterns) were used to identify 23 lactic acid bacteria (LAB) isolated fro...

conclusions the results indicate that multidrug-resistant (mdr) strains of salmonella are increasing in number, and fewer antibiotics may be useful for treating s. enterica infections. routine investigation and reporting of antibiotic mics in patients presenting with salmonella infections is suggested. results of the total 19 salmonella spp. isolates recovered by the bactec automated system, al...

An automated ribotyping device (RiboPrinter) was used to determine the ribotypes of a collection of Burkholderia pseudomallei isolates. In a preliminary evaluation with the restriction enzymes BamHI and EcoRI, the protocol with EcoRI was more discriminating. The reproducibilities of the ribotypes obtained with EcoRI (EcoRI ribotypes) were determined by testing three levels of bacterial loads. T...