Carbohydrates have been suggested to account for some IgE cross-reactions between various plant, insect, and mollusk extracts, while some IgG antibodies have been successfully raised against plant glycoproteins. A rat monoclonal antibody raised against elderberry abscission tissue (YZ1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-li...

background: celiac disease is one of the malabsorption syndromes leads to growth and development retardation in children. there is no test lonely can definitely diagnose celiac; however, the collection of clinical findings, serologic tests, intestinal biopsy, and response to treatment may diagnose it. although diagnostic value is variable in different studies, they are used a non-invasive and a...

background worldwide, the incidence of inflammatory bowel disease (ibd) is increasing. this study aims to evaluate the diagnostic value of two serological markers, atypical perinuclear anti-neutrophil cytoplasmic antibodies (atypical-p-anca) and anti-saccharomyces cerevisiae antibodies (asca), with the intent to determinetheir relationship to ulcerative colitis (uc) and crohn's disease (cd), in...

We describe a solid-phase adsorbent enzyme-linked immunoassay for measuring the amount of antibody in serum. The assay consists of adsorbing an excess of DNA to a polystyrene test-tube support, reacting the anti-DNA serum with the coated tube, following with an additional reaction of an anti-human -y-globulin peroxidase enzyme conjugate with the coated tube, and finally developing a colored rea...

background: zoonotic cutaneous leishmaniasis (zcl) is an increasing public health problem in some endemic re­gions. horseradish peroxidase (hrp) conjugated rabbit anti- rhombomys opimus ( r. opimus ) ig is needed for im­munoblotting and elisa tests used to explore the immune response of the rodents against the sand fly saliva. in this study, the production of hrp conjugated rabbit anti- r. opim...

Autoimmune thyroiditis is one of the most common causes acquired hypothyroidism in children and adolescents. Both genetic susceptibility environmental factors play a role etiopathogenesis. We report two cases autoimmune here with varied presentation. them had different clinical features at On investigation, both elevated thyroid-stimulating hormone (TSH), low T4 anti-Thyroid peroxidase (TPO) an...

We made 202 examinations on specimens from 78 patients, to evaluate the application of horseradish peroxidase-labeled antisera as compared to fluorescein-labeled material in a clinical immunology laboratory. Various circulating and tissue-fixed antibodies were identified by both techniques. The horseradish peroxidase technique can be substituted for the fluorescein isothiocyanate method for the...

Studies of our experiment are related to the binding of enzymes to antibodies. This involves the formation of a stable, covalent linkage between an enzyme & antibody. We used different Enzymes for this studies which are altered in binding action. Enzymes we used-e.g. Horseradish peroxidase (HRPO), urease, or alkaline phosphatase. Studies of Enzyme and an antigen-specific monoclonal or polyclona...

An immunohistochemical procedure for the detection of immunoglobulin G adherent to platelets is described. The peroxidase anti-peroxidase method is used to detect antibody activity directed against platelets from normal donors in the sera from 305 individuals. These subjects were divided into three groups: group 1, patients referred for tissue typing; group 2, healthy normal females; group 3, h...