Malaria parasite (Plasmodium spp.) infection in the mosquito Anopheles stephensi induces significant expression of A. stephensi nitric oxide synthase (AsNOS) in the midgut epithelium as early as 6 h postinfection and intermittently thereafter. This induction results in the synthesis of inflammatory levels of nitric oxide (NO) in the blood-filled midgut that adversely impact parasite development...

James R. Duncan, MD, PhD, Stephen Balter, PhD, Gary J. Becker, MD, Jeffrey Brady, MD, MPH, James A. Brink, MD, Dorothy Bulas, MD, Mythreyi B. Chatfield, PhD, Simon Choi, PhD, MPH, Bairbre L. Connolly, MB, Robert G. Dixon, MD, Joel E. Gray, PhD, Stephen T. Kee, MD, Donald L. Miller, MD, Donald W. Robinson, LTC, MD, Mark J. Sands, MD, David A. Schauer, DSc, Joseph R. Steele, MD, Mandie Street, RT...

Title of Dissertation: GENETIC MANIPULATION OF ANOPHELES STEPHENSI IMMUNITY TO INCREASE PLASMODIUM FALCIPARUM SALIVARY GLAND SPOROZOITE INFECTION LEVELS Kasim I. George, Doctor of Philosophy, 2016 Dissertation directed by: Professor David A. O’Brochta, Institute for Bioscience & Biotechnology Research Department of Entomology Human malaria is responsible for over 700,000 deaths a year. To stay ...

Staphylococcus hominis is a culturable component of the bacterial microbiome of Anopheles stephensi. Here, we present the annotated draft genome sequences of three S. hominis isolates from A. stephensi. These genomic resources will facilitate experiments to further our understanding of the role of bacteria in mosquito biology.

Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The continued use of synthetic insecticides has resulted in resistance in mosquitoes. Synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternati...

BACKGROUND Mosquitoes transmit serious human diseases, causing millions of death every year. Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. Nine different locally available medicinally important plants suspected to posse larvicidal property wer...

Anopheles (Cellia) stephensi Liston is a major vector species of malaria in Indian subcontinent. Taxonomists have worked on its various morphological aspects and immature stages to explore additional and new taxonomic attributes. Scanning electron microscopic (SEM) studies have been conducted on the fourth instar larva and pupa of An. stephensi to find additional taxonomic features for the firs...

Strains of Serratia marcescens, originally isolated from the gut lumen of adult female Anopheles stephensi mosquitoes, established persistent infection at high rates in adult A. stephensi whether fed to larvae or in the sugar meal to adults. By contrast, the congener S. fonticola originating from Aedes triseriatus had lower infection in A. stephensi, suggesting co-adaptation of Serratia strains...

Bioinsecticide Bacillus thuringiensis var. kurstaki (Btk) was used for controlling the mosquito species (Anopheles stephensi and Culex quinquefasciatus) which gave a significant (p<0.05) mortality in both species. The higher concentration of Btk was highly effective compared to the control ones. The controlling effect was dose and time dependent. Among the studied mosquitoes the C. quinquefasci...

Petroleum ether extract of Blumea lacera was screened under laboratory conditions for repellent activity against mosquito vector Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae). The repellent activity of Blumea lacera extract was tested against mosquitoes in comparison with the DEET, which was used as a positive control. Results obtained from the laboratory experi...