The influence of thawing procedures on fertility of semen in .25-ml Continental 3 straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<...

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...

cryopreservation is as an effective way to prevent species extinction risk. in this study, we have investigated the protective effect of different concentrations cryoprotectant and effect of different dilution ratios on sperm motility percent and its motility duration after thaw. for this work dimethyl sulfoxide, methanol and ethylene glycol was used as cryoprotectant, each at concentration of ...

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen wa...

Semen obtained from four Beluga males (Huso huso) was cryopreserved using extender; Tris-sucroseKCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) supplemented with MeOH at concentration of 5%, 10% and 20%. Semen was diluted, respectively, with ratios of 1:0.5, 1:1 and 1:2 with extender and frozen in liquid nitrogen vapor.Frozen sperms after 30 and 60 days were excluded from freezing. Experime...

The characteristics of male and female reproductive tracts and reproductive physiology in camelids are described. An account is given on methods of collection, characteristics and storage of semen, and fertility after artificial insemination (AI) with fresh, liquid-stored and frozen-thawed lamoid and camel semen.