D epartm ent of Radiology. University of California, San Francisco, California 94143, USA Z. Naturforsch. 41b, 1170—1177 (1986); received February 20, 1986 Bovine Serum Albumin, Gadolinium Complexes, Contrast Agents for NMR Imaging, Nitroxyls Bovine Serum Albumin (BSA) was spin labeled using the active esters 3 and 5 containing a nitroxyl radical to give the spin labeled BSA derivatives 4 and 6...

The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

the aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºc for 0, 12, 24, 48 and 72 hrs after collection anddilution. a total of 57 ejaculates from 12 bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

the interaction of bovine serum albumin ( bsa) and egg albumin with water solublephthalocyanine, cobalt (ιι) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( cotspc) , has been studiedby the uv- vis method at ph 7.0 and five different temperatures 20, 25, 30, 35 and 40°c. theformation constants have been elucidated by using spectrophotometric titration and computersquad program data refinement. th...

OBJECTIVE To evaluate (1) to what extent sera from healthy subjects and patients with rheumatoid arthritis (RA) contain antibodies to bovine serum albumin (BSA); and (2) if anti-BSA antibodies interfere with results of enzyme-linked immunoassays (ELISA) containing BSA. METHODS The ELISA used was a previously developed in-house assay of autoantibodies to tumor necrosis factor (TNF). Anti-TNF a...

Antibody  based  methods  have  numerous  advantages  compared  to  other  detection  methods. However, heavy metals cannot stimulate the immune antibody response; it is the main obstacle for preparation of the antibodies that used in detection methods of the metals. The production of the immunogenic Pb complexes by using cost benefit linkers of EDTA and DTPA were investigated in this  study.BS...

The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...

Immunofluorescence Cells were fixed in 2% (v/v) formaldehyde for 40 min. After being washed in PBS, the cells were permeabilized in a blocking buffer containing 10% (v/v) FBS, 3% (w/v) BSA and 0.5% (v/v) Triton X-100 in PBS. The cells were then incubated at 4 °C with primary antibodies against EEA1, CI-MPR, clathrin heavy chain or the α subunit of AP-2 for 6-12 hours. Excess antibody was remove...

The present study examined the factors responsible for triggering renal hemodynamic adjustments during acute volume expansion. The renal hemodynamic effects of graded volume expansion with 0.9% saline (Sal; 1, 2, and 4% of body wt), 7% BSA solution (0.35, 0.70, and 1.4% body wt), or whole blood from a donor rat (WBL; 0.35, 0.70, and 1.4% body wt) were compared in rats anesthetized with pentobar...