To evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (Melophagus ovinus) were collected from 23 sheep, 144 deer keds (Lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. DNA was extracted individually or in ...

Several tick-borne diseases with different etiological agents such as rickettsia, protozoa, bacteria and viruses can infect domestic ruminants. The only common feature between these diseases is that they can all be transmitted by ticks. Of these, Anaplasma spp. can cause diseases in the livestock with high economical losses. The Giemsa staining of blood smear is the common traditional method fo...

ovine anaplasmosis is a tick-borne rickettsial disease, widespread in tropical and subtropical areas. in the present study, a pcr-rflp method based on major surface protein 4 (msp4) gene, was utilized for the detection of anaplasma infection in 119 sheep blood samples collected from different parts of ahvaz in the southwest of iran. pcr identified anaplasma infections in 87.4% (104/119) of the ...

Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma sp...

The genus Anaplasma includes a diverse group of tick-borne pathogens found exclusively within membrane-bound vacuoles in host cells. While A. marginale, A. centrale and A. ovis, vectored by Dermacentor and Rhipicephalus ticks, are host-specific for ruminants, A. phagocytophilum, vectored by Ixodes spp., infects a wide range of hosts. In ticks Anaplasma undergoes a developmental cycle that is co...

Krajem svibnja 2015. godine u Laboratorij za parazitologiju, Hrvatskog veterinarskog instituta, Zagreb, Hrvatska dostavljena je krv ovna iz okolice Benkovca sa sumnjom na piroplazmozu. Ovan uz potpornu terapiju vitaminima AD3E i B-kompleka liječen imidokarbom (Imizol 0,5 mL s/c) oksitetraciklinom (Geomicin retard 5 i/m) te pet dana prestao pokazivati kliničke znakove bolesti. U razmazu krvi pro...

Anaplasmosis, a disease caused by various species of Anaplasma, poses important economic constraints to animal breeders. Ehrlichiosis is a worldwide zoonosis illness and mostly occurs in tropical and subtropical regions that are close to the vector’s distribution. Tick-borne pathogens lead to over 100,000 cases of illness in the world each year. Besides the costs of the additional veterinary ca...

To the Editor: Anaplasmosis is a disease caused by bacteria of the genus Anaplasma. A. marginale, A. centrale, A. phagocytophilum, A. ovis, A. bovis, and A. platys are obligate intracellular bacteria that infect vertebrate and invertebrate host cells. A. ovis, which is transmitted primarily by Rhipicepha-lus bursa ticks, is an intraerythrocytic rickettsial pathogen of sheep, goats, and wild rum...

A commercially available (cELISA) kit for diagnosing Anaplasma marginale infection in cattle was validated for diagnosing A ovis infection in sheep using the bovine serum controls as supplied by the manufacturer (BcELISA) and sheep serum controls from pathogen-free sheep (OcELISA). True positives were identified using two previously established assays, a nested PCR (nPCR) test and an indirect i...

Geographical sero-prevalence of Anaplasma marginale (T) in sheep, Ovis aries (L) was done from January-May, 2012 in district Peshawar which is a crowded area of Pakistan. In this area sheep’s infection with A. marginale is not reported before. For this purpose, 376 serum samples were obtained conveniently from 4 different breeds of sheep, from different geographical areas of Peshawar. An indire...