An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a sin...

The current study deals with a digestive α-amylase in the larvae of Pieris brassicae L. through purification, enzymatic characterization, gene expression, and in vivo effect of a specific inhibitor, Acarbose. Although α-amylase activity was the highest in the whole gut homogenate of larvae but compartmentalization of amylolytic activity showed an equal activity in posterior midgut (PM) and ante...

A bifunctional alpha-amylase/subtilisin inhibitor (RASI) was purified to electrophoretic homogeneity from rice (Oryza sativa L.) bran. Its molecular mass was 21 kDa by SDS-PAGE and its isoelectric point was 9.05. Purified RASI inhibited subtilisin Carlsberg strongly and inhibited alpha-amylase from germinating rice seeds weakly. It inhibited rice alpha-amylase more than barley alpha-amylase, an...

An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK) was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optim...

The present study was aimed at purification and characterization of α-amylase enzyme from strain of Aspergillus niger isolated from local soil samples. Solid state fermentation (SSF) was carried out to produce α-amylase from A. niger using waste banana peel as substrate. The maximum activity of α-amylase (11926 U/gds) was recorded after 72 hours of fermentation. The extracted enzyme was subject...

BACKGROUND There is increasing evidence that chaperones are also present outside the cell, exerting cytokine-like effects and influencing immune recognition. Hsp70 has been found to be present in human blood sera. Chaperonins Cpn10 and Cpn60 are present in pancreatic juice, but Hsp70 is not. These observations raise the possibility that molecular chaperones may be present in other secretory flu...

The impact of ultra violet radiations on alpha amylase producing ability of Bacillus subtilis (2620) was studied. Maximum enzymatic activity (crude) upto 17 folds were obtained from mutant strain exposed to 60 minutes ultra violet irradiation in comparison to parent strain. Purified enzyme showed molecular weight of 68 KDa and 54 KDa by SDS-PAGE in both parent and mutant strains. The SDS-PAGE o...

the experiment was conducted to determine α-amylase activity and the effect of seven plant species extracts including punica granatum l. (punicaceae), rheum officinale b. (polygonaceae), rhus coriaria l. (anacardiaceae), artemisia sieberi b. ( compositae), peganum harmala l. (nitrariaceae) , datura stramonium l. (solanaceae) and thymus vulgaris l. (lamiaceae) on α-amylase activity of four store...

the experiment was conducted to determine α-amylase activity and the effect of seven plant species extracts including punica granatum l. (punicaceae), rheum officinale b. (polygonaceae), rhus coriaria l. (anacardiaceae), artemisia sieberi b. ( compositae), peganum harmala l. (nitrariaceae) , datura stramonium l. (solanaceae) and thymus vulgaris l. (lamiaceae) on α-amylase activity of four store...

An interesting α-amylase has been obtained in large quantity (2400 U/g of body weight) with specific activity (20.22 U/g protein) from a freshwater zooplankton, Heliodiaptomus viduus (Gurney). Partially purified enzyme showed activity up to 70 oC and demonstrated optimum activity at 30 oC. The enzyme was active between pH 3.5 and 8.5, with maximum activity at pH 6.0. It retained its full activi...