Abstract-HCTN7. In this paper, antidiabetic activities of glucomanno-oligosaccharides (GMO) in vitro and vivo were investigated. GMO significantly increased AMP-activated protein kinase (AMPK) phosphorylation a concentration-independent manner. Treatment with 100μg/ml 50μg/ml for 1 hour caused 1.47-fold 1.81-fold AMPK, respectively. Oral administration (6g/kg-1 body weight day-1) lowered blood ...

Heart failure is one of the leading causes death and disability worldwide. Left ventricle remodeling, fibrosis, ischemia/reperfusion injury all contribute to deterioration cardiac function predispose onset heart failure. Adenosine monophosphate-activated protein kinase (AMPK) universally recognized energy sensor which responds low ATP levels restores cellular metabolism. AMPK activation control...

The metabolic sensor AMP-activated protein kinase (AMPK) has emerged as an important link between cellular metabolic status and ion transport activity. We previously found that AMPK binds to and phosphorylates CFTR in vitro and inhibits PKA-dependent stimulation of CFTR channel gating in Calu-3 bronchial serous gland epithelial cells. To further characterize the mechanism of AMPK-dependent regu...

The multifunctional AMPK-activated protein kinase (AMPK) is an evolutionarily conserved energy sensor that plays an important role in cell proliferation, growth, and survival. It remains unclear whether AMPK functions as a tumor suppressor or a contextual oncogene. This is because although on one hand active AMPK inhibits mammalian target of rapamycin (mTOR) and lipogenesis--two crucial arms of...

1. Abstract 2. Introduction 3. Activation of skeletal muscle AMPK by exercise 4. Allosteric and covalent regulation of AMPK activity 5. Nucleotide dependent activation of AMPK 6. Calcium dependent activation of AMPK 7. Muscle glycogen dependent activation of AMPK 8. Reactive oxygen species dependent activation of AMPK 9. TAK1 – a cytokine dependent AMPK regulator? 10. Concluding remarks 11. Ack...

5'-AMP-activated protein kinase (AMPK) is a key regulator of metabolism and survival during energy stress. Dysregulation of AMPK is strongly associated with oxidative-stress-related disease. However, whether and how AMPK is regulated by intracellular redox status remains unknown. Here we show that the activity of AMPK is negatively regulated by oxidation of Cys130 and Cys174 in its α subunit, w...

AMP-activated protein kinase (AMPK) is an evolutionarily conserved heterotrimeric complex composed of α (catalytic α1 and α2) and β/γ (regulatory β1, β2, γ1, γ2, and γ3) subunits and serves as a gatekeeper of energy metabolism by responding to metabolic needs of a cell. Recent findings implicated the enzyme in a broad range of cellular functions, including cell growth, autophagy, polarity, tran...

Muscle contraction results in phosphorylation and activation of the AMP-activated protein kinase (AMPK) by an AMPK kinase (AMPKK). LKB1/STRAD/MO25 (LKB1) is the major AMPKK in skeletal muscle; however, the activity of LKB1 is not increased by muscle contraction. This finding suggests that phosphorylation of AMPK by LKB1 is regulated by allosteric mechanisms. Creatine phosphate is depleted durin...

BACKGROUND AND PURPOSE 5' adenosine monophosphate-dependent protein kinase (AMPK) acts as a metabolic sensor. AMPK is elevated under ischemic conditions, but the role of AMPK in ischemic brain remains controversial. In this study, we examined the effects of AMPK inhibition using both pharmacological and genetic approaches in an in vivo stroke model. METHODS Focal stroke was induced by reversi...