Commercial control sera contain varying amounts of pyridoxal-5'-phosphate. Normalization of aminotransferase activity measurement with enzyme "standards" is possible only when pyridoxal-5'-phosphate is added to the reaction medium. Pyridoxal-5'-phosphate addition should be compulsory in national recommendations.

Regulation of tyrosine aminotransferase gene expression was studied in an adult rat hepatocyte line (RALA255-10G) which is temperature sensitive for the maintenance of the differentiated liver phenotype. Glucocorticoid hormones such as cortisol were necessary for expression of the aminotransferase gene. In the absence of these steroids, enzyme synthesis, activity, and mRNA accumulation were vir...

Species of coryneform bacteria (Corynebacterium glutamicum, Brevibacterium flavum, and B. ammoniagenes) are capable of transaminating all three of the aromatic pathway intermediates; prephenate, phenylpyruvate, and 4-hydroxy-phenylpyruvate. Two molecular species of aromatic aminotransferase (denoted aminotransferase I and aminotransferase II) were partially purified from C. glutamicum and B. fl...

Coffee consumption is a regular part of daily life throughout the world. Research into the effects of coffee on human health is ongoing, but a recent study suggests that coffee and caffeine consumption can reduce the risk of elevated alanine aminotransferase activity in individuals at high risk for liver disease. This review will analyze the results of that study in light of the current literat...

For the PAT application of microbial monitoring instruments in pharmaceutical field, certain performance features of a detector technology are highly desirable, among them are: (1) real time microbial detection; (2) capability of continuous monitoring of microbial level in the environment; (3) broad detection range of microbes; (4) freedom from sample preparation and (5) ease of operation. To p...

We propose a routine method for the mechanized measurement of aspartate aminotransferase with the CentrifiChem Analyzer, which is based on the recommendations of both the International Federation of Clinical Chemistry and the Société Française de Biologie Clinique. A modification of the CentrifiChem pipettor permits simultaneous pipetting of two reagents, thus achieving automatic preincubation ...