The precise sequence of aminoacids plays a central role in the tertiary structure of proteins and their functional properties. The Hydrophobic-Polar lattice models have provided valuable insights regarding the energy landscape. We demonstrate here the isomorphism between the protein sequences and designable structures for two and three dimensional lattice proteins of very long aminoacid chains ...

Appreciated for its exotic flavor and considered a nutrient-rich food, Brazil nut is composed mostly of unsaturated fatty acids and high biological value proteins. The aim of this study was to evaluate the profile of amino acids in Brazil nut powdered milk using an HPLC system. The Brazil nut powdered milk was featured and the aminoacid profile was evaluated. The amino acids values of glutamic ...

From the first analysis of immuneprecipitation followed by Western Blotting (WB) Corin and TLP seem to precipitate at the same height ( approximately 50KDa) and are recognized by the same antibodies. In parallel we are improving the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 with the aim to be able of obtaining a precipitate containi...

From the first analysis of immuneprecipitation followed by Western Blotting (WB) Corin and TLP seem to precipitate at the same height ( approximately 50KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 are improved with the aim to be able of obtaining a precipitate containing o...

We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same stra...

High enantioselectivities and activities are achieved in the Ir-catalyzed hydrogenation of several unfunctionalized olefins using modular biaryl phosphite-oxazoline ligands from hydroxyl aminoacid derivatives; the presence of a biaryl phosphite group is crucial to this success.

A procedure is presented for proline quantification in serum filtrates and urine hydrolysates. Interference from primary amino acids is greatly reduced by nitrozation. After nitrozation and neutralization, protine is reacted with ninhydrin in a concentrated electrolyte solution. The resulting red proline-ninhydrin product is salted out, extracted into benzene, and measured at 520 nm. The extent...