28, 36, and 41 kDa, On the other hand, Kurosawa et al.4) found an alkaline RNase in addition to three acid nucleases in frcsh fruit bodies of L, edodes confirming the results of Endo et aLi) To establish more precise information on these enzymes, we tried to isolate and characterize each nuclease or RNase in L. edodes. First, we isolated an acid RNase, RNase Le2, and established the prirnary st...

Inside Cover: In article number 2000580 by Dagmar Fischer, Kalina Peneva, and co-workers, guanidinium, containing methacrylamide terpolymers with three different side chains mimicking hydrophobic amino acid residues, are designed to evaluate what extent does the identity of these groups influence binding, protection, transfection DNA.

amino acid dehydrogenases (l-amino acid: oxidoreductase deaminating ec 1.4.1.x) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either nad+, nadp+ or fad. these enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

Semliki Forest virus (SFV) infection of mice was used as a model to study the applicability of synthetic peptides containing only linear epitopes as viral vaccines. The identification of linear epitopes with vaccine potential on the E2 membrane protein of SFV was based on the binding of SFV-specific antibodies to a set of overlapping synthetic hexapeptides (Pepscan) representing the whole E2 am...

Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...

Major immunogenic sites of foot-and-mouth disease virus (FMDV) have been mapped to the C-terminal third of capsid protein VP1; we studied the immunogenicity of a series of TrpE-FMDV fusion proteins containing this region of FMDV strain O1 Campos. Fusion protein TrpE-dCN, which contains a dimer of VP1 amino acid sequences consisting of amino acids 200 to 213 linked by a diproline spacer to amino...

The yield and purity of synthetic peptides were greatly related to the amino acid protection and activation during the synthesis process. Therefore, the amino acid protection and activation are the most important steps in peptide synthesis. By using tetrahydrofuran as the solvent, 9-fluorenylmethoxycarbonyl as protection group, 2-(7-azobenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorop...

For the first time we have been able to employ enantiopure 1,2-amino alcohols derived from abundant amino acids in C−C bond-forming hydrogen-borrowing alkylation reactions. These reactions are facilitated by use of aryl ketone Ph*COMe. Racemisation amine stereocentre during can be prevented sub-stoichiometric base and protection nitrogen with a sterically hindered triphenylmethane (trityl) or b...