Bile salts of the coelacanth, Latimeria chalumnae, Smith, have been analyzed and shown to have three bile alcohols, latimerol, 5 alpha-cyprinol, and 5 alpha-cholestane-3 beta, 7 alpha,-12 alpha,25,26-pentol, two C24 bile acids, chenodeoxycholic acid and cholic acid, one C26 bile acid, probably 3 beta, 7 alpha, 12 alpha-trihydroxy-27-nor-5 alpha-cholestan-26-oic acid, and two C27 bile acids, 3 a...

Abstract The Joint Research Centre, in cooperation with the Commissariat à l’Energie Atomique et aux Energies Alternatives, produced a novel 243 Am spike reference material for mass spectrometry. Americium solution an isotopic composition of 88% and 12% 241 was used as source preparation material. certified value 5.696 (11) nmol g −1 amount content 0.136138 (54) n ( Am)/ Am) ratio were assigned...

The present paper describes the individual analytical methodologies (Thermal Ionization Mass Spectrometry, Inductively Coupled Plasma Mass Spectrometry and Alpha Spectrometry) that were developed for uranium age determination and discusses their advantages and drawbacks. With respect to a potential application of the methods (e.g. Fissile Material Cut-off Treaty), the discussion focuses on high...

Applications such as environment monitoring implying alpha emitters activity measurement associated with isotope identification, require high-energy resolution detectors. Conventional silicon detectors are inexpensive therefore widely used, although intrinsically limited in energy resolution. Thermal detection principle of cryogenic detectors introduces a breakthrough in alpha particle measurem...

Metastasis is a serious and often life-threatening condition, representing the leading cause of death among women with breast cancer (BC). Although current clinical classification BC well-established, addition minimally invasive laboratory tests based on peripheral blood biomarkers that reflect pathological changes in body utmost importance. In study, serum proteome lipidome profiles for 50 pat...

3-Amino-3-deoxy-alpha-D-glucopyranosyl-alpha-D-glucopyranoside (alpha, alpha--3-trehalosamine) was isolated from a culture of Nocardiopsis trehalosei sp. nov. (NRRL 12026). The structure was determined using a combination of spectroscopic techniques on derivatives of the component sugars, especially gas chromatography-mass spectrometry. The compound exhibited antibiotic activity against Gram-po...

Mass spectrometry is a rapid, sensitive, and accurate quantitative approach for the direct monitoring of enzyme-catalyzed reactions that does not require a chromophore or radiolabeling and thus provides a viable alternative to existing analytical techniques. In this study the proteolysis of intact viral capsid proteins, the alpha-glucosidase-catalyzed hydrolysis of p-nitrophenyl-alpha-glucopyra...

BACKGROUND Treatments now available for mucopolysaccharidosis I require early detection for optimum therapy. Therefore, we have developed an assay appropriate for newborn screening of the activity of the relevant enzyme, alpha-L-iduronidase. METHODS We synthesized a new alpha-L-iduronidase substrate that can be used to assay the enzyme by use of tandem mass spectrometry together with an inter...

Alpha-synuclein is a major component of Lewy bodies, proteinacious inclusions which are a major hallmark of Parkinson's disease (PD). Lewy bodies contain high levels of nitrated tyrosine residues as determined by antibodies specific for 3-nitrotyrosine (3NT) and via mass spectrometry (MS). We have developed a multiple reaction monitoring (MRM) mass spectrometry method to sensitively quantitate ...

Four positional isomers of sialyloligosaccharides were isolated from the urine of an adult patient with sialidosis with partial deficiency of beta-galactosidase. From structural studies including compositional sugar analysis, exoglycosidase digestion, chemical ionization mass spectrometry, proton nuclear magnetic resonance spectrometry, and methylation analysis, their structures were deduced to...