A whole-cell biocatalyst with the ability to induce synergistic and sequential cellulose-degradation reaction was constructed through codisplay of three types of cellulolytic enzyme on the cell surface of the yeast Saccharomyces cerevisiae. When a cell surface display system based on alpha-agglutinin was used, Trichoderma reesei endoglucanase II and cellobiohydrolase II and Aspergillus aculeatu...

The S-carboxymethyl derivative chains of crosslinked and noncrosslinked fibrins were prepared from purified human fibrinogen. For crosslinked fibrin, fibrinogen was clotted with thrombin in the presence of calcium and purified human factor XIII. For noncrosslinked fibrin, ethylenediaminetetraacetate was substituted for factor XIII and calcium. After reduction with dithiothrcitol and alkylation ...

Maltase activity (EC 3.2.1.20) was solubilized from rabbit kidney brush-border membrane by using 1.0% Triton X-100 and purified 230-fold with an overall recovery of 30%. The purification procedure makes use of heat precipitation, chromatography on DE-52 DEAE-cellulose and gel filtration on Sephacryl S-300. Rabbit kidney brush border exhibited glucoamylase activity with a maltase/glucoamylase ra...

Dardas, T. J. (Michigan State University, East Lansing), and V. H. Mallmann. Electrophoretic and immunoelectrophoretic studies of sera from normal, tuberculous, and noninfected tuberculin-sensitive guinea pigs. J. Bacteriol. 92:76-81. 1966.-Normal guinea pig serum was separated into seven fractions by electrophoresis on cellulose acetate membranes. Thirty antigens were found by immunoelectropho...

Because there is a lack of wood resources in many countries, this work focused on pulp and paper production from the waste agricultural residues non-wood plants. The aimed to nitrate-alkaline black mustard (Brassica Nigra L.) camelina (Camelina Sativa L.). were selected due expanding planted areas these crops Czech Republic. To characterize chemical composition camelina, cellulose, lignin, ash,...

Haematological and biochemical aspects of a new family with heterozygous Hb O Padova [alpha 30(B11) Glu----Lys] is described. This variant has been found on a screening programme of abnormal haemoglobin in cord blood. It was separated by cellulose acetate, isoelectric focusing, citrate agar, DEAE-cellulose chromatography and by cation exchange high-performance liquid chromatography. The heat st...

A cellulose derivative substance called acetate is frequently used in the manufacturing of photographic film, fiber, membranes, and bioplastics. However, availability sources for acetate's raw material does not support this demand. Paper waste has a high content. Therefore, aim research to synthesize from α-cellulose paper. In order separate alpha paper study, delignification process with NaOH ...

Background and Objectives Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the...