The detrimental effects of donor-directed antibodies in sensitized transplant patients remain a difficult immunologic barrier to successful organ transplantation. Antibody removal is often followed by rebound. Proteasome inhibitors (PIs) deplete antibody-producing plasma cells (PCs) but have shown marginal benefit for desensitization. In an allosensitized nonhuman primate (NHP) model, we observ...

BACKGROUND Knowledge of the human leukocyte antigen (HLA) amino acid (AA) sequence combined with crystallographic structural data may enable prediction of the relative immunogenicity of individual donor/recipient HLA mismatches. METHODS Multiple sera from 32 highly sensitized patients awaiting kidney transplantation were screened using Luminex/single-antigen beads to determine the HLA-specifi...

สวนใหญการเตรียมเลือดใหผูปวยมักไมพบปญหาใดๆ จึง ทําาใหผูปวยไดรับเลือดตามตองการ และไมเสียเวลาในการรอ เลือด คงมีผูปวยเพียงสวนนอยเทานั้นที่ตรวจพบวามี positive antibody screening และ/หรือ positive crossmatching ซึ่ง ทําาใหเกิดความลาชาในการใหเลือดแกผูปวย เพราะจะตองทําาการ ตรวจเพิ่มเติมเพื่อหาสาเหตุ การตรวจใชเวลาแตกตางกันขึ้นกับ ชนิดของแอนติบอดีที่ตรวจพบวาเปน alloantibody หรือ autoan...

BACKGROUND We have shown previously that human leukocyte antigen (HLA) class I immunogenicity can be predicted by the number, position, and physiochemical differences of polymorphic amino acids (AAs). We have now modeled the structural and physiochemical polymorphisms of HLA class II alloantigens and correlated these with humoral alloimmunity in sensitized patients awaiting kidney transplantati...

Objective: To identify the trends of red cell alloimmunization in multi-transfused females using blood panels.Study design: Cross-sectional study.
 Place and Duration Study: Army Medical College Armed Forces Institute Transfusion with collaboration Pak Emirates Military Hospital, Rawalpindi Pakistan, from Jan to Aug 2018.
 Methodology: Blood samples 75 prior history transfusion were c...

Evidence is presented to support the contention that IgM demonstrable by surface immunofluorescent staining on H-2-activated T cells represents specifically adsorbed B-cell-derived alloantibody. T cells activated to H-2 determinants expressed surface IgM only when the progenitor cell populations contained B lymphocytes. IgM was not detected on T cells activated to determinants which fail to sti...

Indirect allorecognition occurs when T cells recognize donor MHC presented as peptide epitopes by recipient APC, but the precise nature of the epitopes involved remains unclear. Rejection of rat MHC class I-disparate PVG.R8 (RT1.A(a)) grafts by PVG.RT1(u) (RT1.A(u)) recipients is mediated by indirectly restricted CD4 T cells that provide help for the generation of alloantibody. In this study, e...

BACKGROUND Interleukin (IL)-6 is a regulatory cytokine for T helper type 17 (Th17) and Treg cells and a potent stimulus for B/plasma cells. The current study evaluated the effect of IL-6 receptor (IL-6R) blockade with an antiYIL-6R monoclonal (mMR16-1) in alloantibody recall responses. METHODS A mouse model of human leukocyte antigen (HLA).A2 sensitization was used for studies to evaluate the...