With the use of stigmatic exudate or distilled water as carriers, various antimetabolites, inhibitors, and miscellaneous materials were injected into the hollow styles of detached Lilium longiflorum pistils before, at, or after compatible or incompatible pollination. Pollen tube lengths were measured 48 hr after pollination with pollinated styles incubated at 22-23 degrees C. Substances conside...

in order to study stigma receptivity in peach cultivars : (‘makhmali’, ‘anjiri-ye-tabestane’, ‘anjiri-ye maleki’, ‘haj-kazemi’ and ’zoodras’) controlled pollination was done in both field and laboratory conditions. before pollination trials the in vitro germination of the pollen was tested in petridish. branches with adequate number of flowers were chosen for controlled pollination. self pollin...

In cruciferous plants, self-pollination is prevented by the action of genes situated at the self-incompatibility locus or S-locus. The self-incompatibility reaction is associated with expression of stigma glycoproteins encoded by the S-locus glycoprotein (SLG) gene. Only a few cases of self-compatible plants derived from self-incompatible lines in the crucifer Brassica have been reported. In th...

Self-incompatibility is one of the most important difficulties in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this research identification and screening of 86 almond progenies obtained from selfing Touno after the self-pollination by PCR reaction with specific ...

In the Brassicaceae, intraspecific non-self pollen (compatible pollen) can germinate and grow into stigmatic papilla cells, while self-pollen or interspecific pollen is rejected at this stage. However, the mechanisms underlying this selective acceptance of compatible pollen remain unclear. Here, using a cell-impermeant calcium indicator, we showed that the compatible pollen coat contains signal...

Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20, and 30 min) to explore gene expression during compatible/incompatible pollen-stigma interactions in stigma. Moderate changes of gene expression were...

We have investigated Ca(2+)-involving cell signaling, plasma membrane potentials and conductances and callose formation during early stages of pollination of papillae of Brassica napus. Using fluorescence imaging of calcium green-1, we found that application of a range of pollen types and controls all rapidly produced small localized peaks in papillar cytoplasmic [Ca2+]. This response was more ...

most sweet cherry cultivars are as found as self-incompatible and therefore for commercial fruit production, compatible pollinizers are needed. controlled pollination and microscopic examination of pollen tube growth in the style are two classical methods for determination of compatible and incompatible pollen donor cultivars. this experiment was carried out to explore the compatibility of ‘zar...

This study explored the morphological characteristics and effects of pollination methods on fruit set and characteristics in 4 red pitaya (Hylocereus spp.) clones. The distinctive morphological recognition and classification among pitaya clones were confirmed by the stem, flower and fruit features. The fruit production season was indicated from the beginning of May to the end of August – the be...