The immunoalkaline phosphatase (APAAP) technique was applied to the labelling of decalcified sections of formalin fixed, paraffin wax embedded bone marrow trephine biopsy specimens. A panel of monoclonal antibodies reactive with haemopoietic and epithelial antigens, which survive routine formalin fixation, was assessed on 72 cases of haematological malignancy (including acute and chronic leukae...

BACKGROUND/AIM Chronic myeloid leukemia (CML) represents a malignant myeloproliferative disease developed out of pluripotent hematopoietic stem cell that contains the fusion bcr-abl gene. Disorders that occur in the process of apoptosis represent one of the possible molecular mechanisms that bring about the disease progress. The aim of our study was to carry out the analysis of the presence of ...

Immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. The basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the researcher in samples of normal and/or mali...

OBJECTIVE To assess if the bonding interlayer between the implant and bone in aseptic loosening of total hip replacement (THR) is qualitatively deteriorated by excessive accumulation of anti-adhesive glycoprotein, tenascin-C. METHODS Alkaline phosphatase-anti-alkaline phosphatase (APAAP) method was used for immunohistochemical staining of tenascin-C in interface tissue and control synovial ti...

423 . IAR C Scientific Publi cations No. 34. IARC, Lyon , France, 1982 2 Cordell JL , Falini B, Erber WN, Gos h AK, Abdulaziz Z, McDo nald S, Pullford KAF, Stein H, Maso n DY: Immunoenzyma tic labelling of monoclona l an tibodies using immune complexes of alka line phosphatase and monoclonal an ti-alkaline phosphatase (APAAP complexes). J Histochem Cytoc hem 32:219-229, 1984 3 Krinke G, Nay lor...

The alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical staining technique was used to look for circulating cells of megakaryocyte lineage in peripheral blood smears from 67 cases of myelodysplasia. Small numbers of micromegakaryocytes positive for platelet glycoprotein IIIa were found in 23 cases. These cells superficially resemble small lymphoid cells and are hence diffic...

A panel of seven monoclonal antiepithelial antibodies of different specificities, including anticytokeratin, human milk fat globule membrane, Ca, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differentiation between benign and malignant mesothelial cells and lung carcinoma in h...

A panel of seven monoclonal antiepithelial antibodies of different specificities, including anticytokeratin, human milk fat globule membrane, C, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differentiation between benign and malignant mesothelial cells and lung carcinoma in hi...

423 . IAR C Scientific Publi cations No. 34. IARC, Lyon , France, 1982 2 Cordell JL , Falini B, Erber WN, Gos h AK, Abdulaziz Z, McDo nald S, Pullford KAF, Stein H, Maso n DY: Immunoenzyma tic labelling of monoclona l an tibodies using immune complexes of alka line phosphatase and monoclonal an ti-alkaline phosphatase (APAAP complexes). J Histochem Cytoc hem 32:219-229, 1984 3 Krinke G, Nay lor...