Labile phosphate esters such as phosphocreatine, acetyl phosphate, and ribose-l-phosphate (1) have assumed great biological importance. These esters are so unstable that they are split with great rapidity by the reagents commonly used for inorganic phosphate determination. Therefore, the usual inorganic phosphate measurements in tissue extracts, etc., actually represent the sum of the inorganic...

Carbohydrate units of cell-surface glycoproteins of mouse B- and T-lymphocytes, labelled in their sialic acid residues by the periodate/NaB3H4 method and in their galactose residues by the galactose oxidase/NaB3H4 method after neuraminidase treatment, have been studied. Glycopeptides were prepared from the labelled cells by Pronase digestion and fractionated by concanavalin A affinity chromatog...

Aldolase catalyzes the reversible cleavage of 1 mole of fructose-l ,6diphosphate (HDP) into 1 mole of glyceraldehyde-3-phosphate and 1 mole of dihydroxyacetone phosphate (1, 2). Although the enzyme has been obtained in crystalline form by Warburg and Christian (3) and by Taylor, Green, and Cori (4) only fragmentary data concerning the content of aldolase in different mammalian tissues have been...

Aldolase catalyzes the reversible cleavage of 1 mole of fructose-l ,6diphosphate (HDP) into 1 mole of glyceraldehyde-3-phosphate and 1 mole of dihydroxyacetone phosphate (1, 2). Although the enzyme has been obtained in crystalline form by Warburg and Christian (3) and by Taylor, Green, and Cori (4) only fragmentary data concerning the content of aldolase in different mammalian tissues have been...

The main goal of the work reported here was to determine the degree of oxidative/alkali-labile DNA damages in peripheral blood as well as in the blood stasis from varicose vein of (chronic venous disorder) CVD patients. Moreover, determination of the impact of Detralex usage on the level of (oxidative) DNA damages in CVD patients was evaluated as well.The degree of oxidative DNA damages was stu...

DNA damage caused by methazolastone [an analogue of 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide which does not require metabolic activation] was investigated in L-1210 leukemia which is sensitive to this drug and in a L-1210 subline [L-1210/N,N-bis(2-chloroethyl)-N-nitrosourea (BCNU)] which is resistant to both chloroethylnitrosoureas and methyltriazenes. Both in vitro and in vivo metazo...

The DNA labile sites induced by two nitrosoureas, nimustine (ACNU) and ramustine (MCNU) synthesised in Japan, have been examined in highly reiterated DNA sequences of rat glioma cells. Reiterated fragments of 167 and 203 base pairs (bp), obtained after Hind III and Hae III restriction endonuclease digestion of rat glioma cells DNA, were used as target DNA sequences to determine the labile sites...

Previous research has shown an increase in chromosomal aberrations in patients with worn implants. The type of aberration depended on the type of metal alloy in the prosthesis. We have investigated the metal-specific difference in the level of DNA damage (DNA stand breaks and alkali labile sites) induced by culturing human fibroblasts in synovial fluid retrieved at revision arthroplasty. All si...

A class of acidic nuclear phosphoproteins has been isolated throughout the mitotic cycle and at two points during differentiation in the slime mold Physarum polycephalum. The electrophoretic profiles of these proteins are reproducible and unchanging throughout the mitotic cycle, but reproducible changes occur during differentiation. The proteins are rapidly synthesized after mitosis, and their ...