Isomaltulose is a noncariogenic reducing disaccharide and also a structural isomer of sucrose and is used by the food industry as a sucrose replacement. It is obtained through enzymatic conversion of microbial sucrose isomerase. An Erwinia sp. D12 strain is capable of converting sucrose into isomaltulose. The experimental design technique was used to study the influence of immobilization parame...

This study aimed to determine the effect of the 3D bioprinted gelatin-alginate scaffold on the full-thickness skin wound healing on mouse back and to observe the histopathological changes during the wound healing process. Using a murine wound model, full skin thickness excisions were created on the dorsum of 40 mice. Then, each mouse was randomly assigned to either the control group or treatmen...

α-galactosidase purified from Acinetobacter sp. was immobilized in four different matricesCalcium alginate, Gelatin, Agarose and k-Carrageenan. On comparative analysis, k-Carrageenan immobilized enzyme showed highest immobilization yield of 68% and storage stability for 30 days. Gelatin showed an immobilization yield of 57%. Soluble enzyme led to decrease of 84% in raffinose and 76% in stachyos...

The primary objective of this study was to investigate the survival rate three species encapsulated bacteria (Propionibacterium acidipropionicici, Propionibacterium freudenreichii, and thoenii) in model solutions designed simulate acidity levels found within human gastrointestinal tract. capsules were prepared by extrusion from gelatin sodium alginate a 1:1 ratio on laboratory encapsulator. use...

Floating calcium alginate beads based on gas-generation technique often suffer from poor drug encapsulation and rapid drug release in the acidic environment of the stomach. To overcome the above mentioned demerits, in the present study we have evaluated the influence of formulation factors such as incorporation of mixtures of gasgenerating agents (NaHCO3 and CaCO3), incorporation of gelatin typ...

Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the c...

Lactobacillus casei ATCC 393-loaded microcapsules based on alginate and gelatin had been prepared by extrusion method and the product could increase the cell numbers of L. casei ATCC 393 to be 10(7) CFU g(-1) in the dry state of microcapsules. The microparticles homogeneously distributed with size of 1.1 ± 0.2 mm. Four kinds of microcapsules (S(1), S(2), S(3) and S(4)) exhibited swelling in sim...

The printed Biomaterial Laboratory at UTEP does research a printable hydrogel which can have appropriate properties for tissue engineering of the skin. Skin is the largest organ in our body which protects us from the environment and pathogens. Skin can be affected by burns and also by diabetic foot ulcers. The current tissue engineered skin substitutes for treatment of diabetic foot ulcers have...

Abstract Current in vivo and vitro models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores use of cardiac spheroids (CSs) biofabricate advanced heart. CSs were created from myocytes, fibroblasts endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels then bioprinted on a microelectrode plate drug testing. Bioprint...

Alginate-gelatin mixed gel was applied to immobilized laccase for decolorization of some synthetic dyes including crystal violet. The immobilization procedure was accomplished by adding alginate to a gelatin solution containing the enzyme and the subsequent dropwise addition of the mixture into a stirred CaCl(2) solution. The obtained data showed that both immobilized and free enzymes acted opt...