Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolve...

Gel electrophoresis technique is an indispensable tool in biotechnology and among other related fields for separation of nucleic acids proteins. This study determined the potential selected cassava sweet potato starch DNA as alternative to agarose gel. The sample pH, gelling temperature time were by Light transmittance method proposed Craig et al. [1] Standard procedure was used gel electrophor...

When soluble fractions of rat kidney homogenates were exposed to affinity chromatography columns containing deoxycorticosterone covalently linked to an insoluble agarose matrix, aldosterone-binding activity was reduced by 92%. Similarly, when renal homogenates were exposed to affinity chromatography columns containing estriol linked to agarose, renal estradiol-binding activity was reduced by 93...

MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...

Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.

Gels are widely used for separation of biological molecules by techniques such as gel filtration, ionexchange chromatography, as well as for immobilization of biocatalysts (Armisen 1997; Fuji et al. 2000; Millan et al. 2002). Agarose, one of the neutral gelling substances is widely used not only in the field of biotechnology but also in many industries. Agarose is a linear, neutral marine polys...

An agarose film has been proposed as an efficient substrate for producing microarrays. The original film preparation procedure was simplified significantly by grafting the agarose layer directly onto unmodified microscope glass slides instead of aminated glass slides, and the blocking procedure was replaced with a wash in 0.1x standard saline citrate (SSC) and 0.5% sodium dodecyl sulfate (SDS) ...

Agarose films were formed with the addition of 30 to 70 wt% choline chloride/urea eutectic-based ionic liquid (ChCl/Urea). The ChCl/Urea was prepared through complexation at a 1:2 mole ratio. The films were prepared by dissolving ChCl/Urea in distilled water followed by dispersion of the agarose at 95 °C. The solution was gelled at room temperature, and the formed gel was dried in an oven overn...

This section describes the application of agarose gel electrophoresis to both analytical and preparative separation of DNA fragments. Standard agarose gels separate DNA fragments from ∼0.5 to 25 kb, whereas pulsed-field agarose gels resolve molecules from ∼10 to >2000 kb. Descriptions of standard and pulsed-field agarose gel electrophoresis as well as parameters affecting resolution of large DN...