background: aflatoxin contamination of food and feed stuff is a serious health problem and significant economic concerns. in the present study, the inhibitory effect of candida parapsilosis ip1698 on mycelial growth and aflatoxin production in aflatoxigenic strains of aspergillus species was investigated. methods: mycelial growth inhibitions of nine strains of aflatoxigenic and non-aflatoxigeni...

High-performance liquid chromatography (HPLC) provides a quick and efficient tool for accurately characterizing aflatoxigenic non-aflatoxigenic isolates of Aspergillus flavus. This method also quantitative analysis AFs in The method’s recovery was assessed by spiking mixture AF at different concentrations to the testing medium. validity confirmed using strains A. HPLC system, coupled with fluor...

The aim of the present investigation was to check natural aflatoxin contamination and aflatoxigenic potential of Aspergillus flavus strains isolated from chilgoza pine nuts. Fifty eight samples of chilgoza pine nuts, randomly purchased from retail shops and local markets of different locations of Jammu, were subjected to mycological analysis for isolation of Aspergillus flavus isolates using st...

Contamination of corn, cotton, peanuts and tree nuts by aflatoxins is a severe economic burden for growers. A current biocontrol strategy is to use non-aflatoxigenic Aspergillus flavus strains to competitively exclude field toxigenic Aspergillus species. A. flavus K49 does not produce aflatoxins and cyclopiazonic acid (CPA) and is currently being tested in corn-growing fields in Mississippi. We...

Aflatoxins are among the most powerful carcinogens in nature. The major aflatoxin-producing fungi are Aspergillus flavus and A. parasiticus. Numerous crops, including peanut, are susceptible to aflatoxin contamination by these fungi. There has been an increased use of RNA interference (RNAi) technology to control phytopathogenic fungi in recent years. In order to develop molecular tools targeti...

The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillu...

The pre-harvest biocontrol approach currently used includes laboratory inoculations using non-aflatoxigenic strains of Aspergillus flavus. This strategy effectively suppresses the indigenous aflatoxigenic and reduces aflatoxin accumulation in sweetcorn. current vitro study’s main objective is to determine diametric growth rates both Aflatoxin (AF)+ AF− improve understanding competitive relation...