AIMS To determine the prevalence of Aeromonas spp in the faeces of children and the association with symptoms of gastroenteritis. METHODS Faecal specimens (n = 1026) were cultured for Aeromonas spp using three selective media and an enrichment broth at both 30 degrees C and 37 degrees C. The isolation of Aeromonas spp was correlated with symptoms of gastroenteritis, previous antibiotic use, a...

The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene...

Samples from the kidney of 100 diseased fish with signs of haemorrhagic septicemia and 50 samples from outlet water of fish farms were taken aseptically and cultured. In the laboratory, 75 colonies of gram-negative bacteria were biochemically diagnosed as motile aeromonads in our Bacteriology Laboratory Unit using API 20E rapid identification system. The genotype identification using specific p...

We evaluated the extent to which serogroup O:11 Aeromonas strains could be recovered from both clinical and environmental specimens and the cultural parameters that affected the phenotypic marker (autoagglutination) associated with this group. Of over 200 Aeromonas strains screened, serogroup O:11 was identified only among the phenospecies A. hydrophila and A. sobria and was associated with cli...

A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasmic proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our resul...

A collection of 120 strains isolated from stool specimens collected from humans suffering from gastroenteritis and from environmental samples were analyzed by random amplified polymorphic DNA PCR (RAPD), repetitive extragenic palindromic PCR (REP-PCR), and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). Species of Aeromonoas hydrophila, A. bestiarum, A. salmonicida, A. ...

Two types of flagella are responsible for motility in mesophilic Aeromonas strains. A polar unsheathed flagellum is expressed constitutively that allows the bacterium to swim in liquid environments and, in media where the polar flagellum is unable to propel the cell, Aeromonas express peritrichous lateral flagella. Recently, Southern blot analysis using a DNA probe based on the Aeromonas caviae...

"Alheira" is a traditional smoked meat sausage produced in the north of Portugal, representing an important economic resource for the region. This meat product has been subjected to research studies with the aim of detecting the presence of common foodborne pathogens, but, to our knowledge, isolation of emerging foodborne Aeromonas from alheira has never been previously described. Present work ...

Introduction The question of bacterial culture sensitivity for individual versus pooled samples was discussed at the 2001 Fish Health Biologist Meeting in Tucson, AZ, as procedures for the USFWS Handbook were being developed. The USFWS Handbook strives to conform with methods specified by the Office International des Epizooties (OIE 2000) and the American Fisheries Society (AFS) Blue Book (Thoe...