in this study, 120 accessions of aegilops crassa collected from various geographical areas of iran were analyzed with respect to genome size and protein markers. a flow cytometry survey of these accessions revealed that one hundred and thirteen of the accessions were tetraploid and seven were hexaploid. moreover, these accessions revealed variations in high molecular weight glutenin subunit com...

in order to determine the cytotypes of 67 accessions of aegilops crassa from iran, two flow cytometry and cytogenetics methods were applied. the nucleus of the young leaves was colored using dapi and the intensity of fluorescent determined by flow cytometry. by comparing the intensity of the fluorescent of the ae. crassa samples with ae. tauschii (as an standard sample), two cytotypes 4x and 6x...

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Various digests of (32)P-labelled DNA were examined by two-dimensional ionophoresis on cellulose acetate and DEAE-cellulose paper. The products from digestion with pancreatic deoxyribonuclease and Neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. Ionophoresis on ...

crassa are catalysed by a multienzyme complex (Giles et al., 1967). If this arom multienzyme complex is purified rapidly in the presence of proteinase inhibitors, it is found to be composed of two subunits ofmol.wt. 165000 (Lumsden & Coggins, 1977; Gaertner & Cole, 1977). The genes that code for the five enzyme activities are clustered and for two of the activities allelic complementation has b...

In the post-genome era, insufficient functional annotation of predicted genes greatly restricts the potential of mining genome data. We demonstrate that an evolutionary approach, which is independent of functional annotation, has great potential as a tool for genome analysis. We chose the genome of a model filamentous fungus Neurospora crassa as an example. Phylogenetic distribution of each pre...