Lactose-sensitive fimbriae were identified as the only fimbriae present on Actinomyces naeslundii WVU45 (ATCC 12104). A single antigen reactive with antiserum against WVU45 cells was detected by cross immunoelectrophoresis of isolated fimbriae, and a monospecific antiserum against this antigen reacted with all fimbriae observed on the bacterial surface by immunoelectron microscopy. Moreover, th...

Phagocytosis of Actinomyces viscosus T14V and A. naeslundii WVU45 by human polymorphonuclear leukocytes in the absence of antibody or complement was mediated by the lectin associated with the type 2 fimbriae of these bacteria. This effect was markedly enhanced by exogenous sialidase, an enzyme also secreted by these actinomyces. Since sialidase treatment of the bacteria did not result in increa...

Chronic canaliculitis is an uncommon disease with a protracted course. Its management is prolonged and difficult. An elderly lady was diagnosed to have left lower chronic purulent canaliculitis. Conservative treatment alone did not effect a complete cure. Canaliculotomy with removal of sulphur granules and canalicular curettage also had to be performed to achieve a permanent cure. Unlike many c...

The nucleotide sequence of the chromosomal DNA flanking the Actinomyces naeslundii (formerly A. viscosus) T14V type 1 fimbrial structural subunit gene (fimP) was determined. Six open reading frames (ORFs), in the order 5' ORF3, ORF2, ORF1,fimP, ORF4, ORF5, ORF6 3', were identified. ORF1 encoded a protein of 408 amino acid residues (Mr = 39,270) and had significant sequence homology with the A. ...

The type 1 fimbriae of Actinomyces naeslundii T14V mediate adhesion of this gram-positive species to the tooth surface. The present findings show that the locus for type 1 fimbria production in this strain includes three genes, fimQ for a minor fimbrial subunit that appears to be an adhesin, fimP for the major structural subunit, and srtC1 for a type 1 fimbria-specific sortase involved in the a...

Bergey (1907) is generally credited with the fir'st isolation of Actinomyces from the normal human mouth. Since then there have been a few reports of the recovery from the normal oral cavity of organisms which appear to have been identical, or nearly so, with strains isolated from clinical actinomycosis (Emmons, 1935, 1936, 1938; Ennever et al., 1951; Miller and Drake, 1951; Morris, 1954; Roseb...

Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium through acid-base reactions. The aim of this study was to evaluate the antibacterial cytotoxic properties Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced turbidity colony-forming units Streptococcus mutans Actinomyces naeslundii, compared calcium-fluoroalum...