BACKGROUND & OBJECTIVES Carbapenem-resistant Acinetobacter spp. have gained increasing significance as opportunistic pathogens in hospitalized patients. Carbapenem resistance is often associated with the loss and/or decrease in outer membrane proteins (OMP) and overexpression of multidrug efflux systems. However, carbapenem-hydrolysing beta-lactamases of Ambler Class B (metallo-enzymes) and Amb...

Single- and multispecies biofilms formed by six drinking water-isolated bacterial species were used to assess their susceptibilities to sodium hypochlorite (SHC). In general, multispecies biofilms were more resistant to inactivation and removal than single biofilms. Total biofilm inactivation was achieved only for Acinetobacter calcoaceticus single-species biofilms and for those multispecies bi...

S. Montaña, R. Cittadini, M. del Castillo, S. Uong, T. Lazzaro, M. Almuzara, C. Barberis, C. Vay and M. S. Ramírez 1) Instituto de Microbiología y Parasitología Médica (IMPaM, UBACONICET), Facultad de Medicina, Universidad de Buenos Aires, 2) Sanatorio Mater Dei, 3) Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y ...

Multiply antibiotic-resistant Acinetobacter baumannii isolate D36 was recovered in Australia in 2008 and belongs to a distinct lineage of global clone 1 (GC1). Here, we present the complete 4.13 Mbp genome sequence (chromosome plus 4 plasmids), generated via long read sequencing (PacBio).

To investigate the presence and structure of AbaR-type genomic islands (GIs) in non-Acinetobacter baumannii isolates, a total of 155 non-baumannii Acinetobacter isolates from a South Korean hospital were analyzed. GIs were found in three Acinetobacter nosocomialis and two Acinetobacter seifertii isolates. Their structures were similar to those in A. baumannii isolates from Asian countries, incl...

Biosurfactants produced by an isolated thermophilic strain Acinetobacter calcoaceticus BU03 were demonstrated to be effective in enhancing the solubility of polycyclic aromatic hydrocarbons (PAHs) and the present study aimed at investigating its effectiveness in increasing bioavailability of PAHs in soil for biodegradation under thermophilic composting condition. At 25 times of its critical mic...

Acinetobacter baumannii is an emerging multidrug-resistant nosocomial pathogen. Bacteriophages may be useful as an alternative method of treatment against this and other multidrug-resistant bacteria. Here, we present the complete genome sequence of A. baumannii phage Presley, an N4-like podophage.

We describe an in vivo evolution of an antimicrobial profile from susceptibility to full-resistance to carbapenems, with heteroresistance as an intermediate stage, in an Acinetobacter baumannii strain. Heteroresistance was characterized by the growth of sub-populations within the susceptibility halo in both disk-diffusion and Etest. PCRs for the main A. baumannii carbapenemases were negative. T...

RP4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in Acinetobacter calcoaceticus EBF65/65. The resulting genetic map indicated that the chromosomal organization of A. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to Pseudomonas in showing an absence of clustering of functionally related genes.

We report here the 4.1-Mb draft genome sequence of Acinetobacter baumannii strain 5021_13, a cerebrospinal fluid isolate from northern India. This genome information will help studies toward understanding the epidemiology and pathogenicity of this important nosocomial pathogen.