Airway hyperresponsiveness (AHR) might be driven by mechanisms inherent to the airway wall, and/or by factors arising from outside the airways. A porcine model of allergen-induced AHR was utilized to investigate physiological responses in intact airways in vitro and their contribution to responsiveness in vivo. Responsiveness to acetylcholine (ACh) was measured in eight ovalbumin (OA)-sensitize...

We used patch-clamp recordings and fast optical Ca(2+) imaging to characterize an acetylcholine-induced current (I(ACh)) in leg motoneurons of the stick insect Carausius morosus. Our long-term goal is to better understand the synaptic and integrative properties of the leg sensory-motor system, which has served extremely successfully as a model to study basic principles of walking and locomotion...

We investigated the involvement of calcium-dependent enzymes, protein kinase C (PKC) and calcium-calmodulin-dependent protein kinase II (CaMKII), in the signaling pathway triggered by the activation of presynaptic alpha7-type nicotinic acetylcholine receptors by exogenous choline, leading to downregulation of the evoked acetylcholine (ACh) release in mouse motor synapses. Blockade of PKC with c...

ABSTRACT The objectives of this study were to investigate the effects different concentrations Suaeda rigida polysaccharides (SRPs) on physiological characteristics frog heart and gastrocnemius muscle, compare their similarities differences, analyze mechanisms. CaCl2 acetylcholine (Ach) selected respectively be co-incubated with high concentration SRPs observe contraction frog. activities acety...

background and aims: the antioxidant and anti-inflammatory effects of carvacrol were reported. the aim of the current study was an investigation the relaxant effects of carvacrol on acetylcholine-induced contraction in isolated rat's ileum. materials and methods: in this study, the tissues separated from ileum were fixed on the organ bath containing tyrode's solution. the amplitude of...

We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) bindi...

Few data have suggested how norepinephrine (NE) and acetylcholine (Ach) regulate the development of Leydig cells in mice at prepuberty, except for data indicating endocrine effects. The present study aims to elucidate the roles of NE and Ach on the differentiation and proliferation of Leydig cells. Firstly, the expression of adrenergic receptors and muscarinic acetylcholine receptors in Leydig ...

Abstract Background Thermodilution-derived measurement of absolute coronary flow and resistance has been recently validated by using a dedicated microcatheter pressure-temperature guidewire. Coronary (AbsF) derived (AbsR) can give precious information about the microcirculation in patients with ischemia non-obstructive artery disease (INOCA). The correlation different INOCA endotypes so far poo...

Pleurotus pulmonarius crude aqueous (CA) extract was previously reported to have therapeutic potential, thus its ability alleviate serum total cholesterol, vasodilation, and improve the aortic tissue structure in hypercholesterolemia rat model studied. Eight groups of Wistar-Kyoto rats were involved including normal (G1), (G2), treatment (G3 G5) prevention (G6 G8). Two doses CA; 0.5 g/kg body w...

The aim of the study was to assess post-harvest life leaves Hemerocallis × hybrida ‘Agata’, Limonium latifolium, and Heuchera ‘Chocolate Ruffles’ after application growth regulators from group gibberellins (GAs) cytokinins (CKs), ionic liquids (2-hydroxyethyl)dimethylethylammonium gibberellinate [Chol][Gib] acetylcholine [Gib][Ach]), as well quaternary ammonium salts with anion (1-ethyl quinine...