The interaction of stearoyl-(1,N6)-etheno coenzyme A (stearoyl-epsilon-CoA) with acetyl coenzyme A carboxylase was investigated by using fluorescence spectroscopy. The fluorescence emission of stearoyl-epsilon-CoA was partially quenched by acetyl coenzyme A carboxylase. Analysis of the data for dissociation constant (KD) and the stoichiometry of the interaction (n) gave values of 5.06 nM and 1....

Acetyl-CoA carboxylase catalyzes the first committed step in the synthesis of fatty acids. Because fatty acids are required during myelination in the developing brain, it was proposed that the level of acetyl-CoA carboxylase may be highest in embryonic brain. The presence of acetyl-CoA carboxylase activity was detected in chick embryo brain. Its activity varied with age, showing a peak in the 1...

The complete amino acid sequence of acetyl-CoA carboxylase from chicken liver has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by Edman degradation of peptide fragments obtained by digestion of the enzyme polypeptide with Achromobacter proteinase I or staphylococcal serine proteinase. Chicken liver acetyl-CoA carboxylase is ...

The roles of synthesis and degradation in the regulation of acetyl coenzyme A carboxylase levels in rat liver following fasting and fat-free feeding have been studied. Antibodies prepared to homogeneous chicken liver acetyl-CoA carboxylase were shown to cross-react with rat liver acetyl-CoA carboxylase. Enzyme from both species was totally inactivated as well as precipitated by this antibody pr...

The enzymes of fatty acid synthesis from liver and brain in normal and Blz-deprived rats were studied. Both total and specific activities of fatty acid synthetase and acetyl coenzyme A carboxylase were 2to 5-fold greater in Blz deprivation than in the normal state. The presence of excess activators in the BIp-deprived rat or an excess inhibitor in the normal rat was not found by serial admixtur...

The dietary regulation of fatty acid synthesis de novo in two transplantable hepatomas, 7777 and 96188, has been studied by comparison of the acetyl coenzyme A carboxylase and fatty acid synthetase activities of tumor and host liver, both in animals fasted 48 hours and in those subsequently refed a fat-free diet for 48 hours. Neither acetyl-CoA carboxylase nor fatty acid synthetase of these hep...

The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-met...

heat-stable protein kinse inhibitor (50 units/ml), therefore ruling out the possibility that the protein kinase represents the free catalytic subunit of cyclic AMP-dependent protein kinase. Heparin, at a concentration of IOpg/ml, which inhibits the phosphorylation of acetyl-CoA carboxylase by casein kinase I and I1 (Tipper et al., 1983; Munday & Hardie, 1984) also had no effect on the phosphory...

The dietary regulation of fatty acid synthesis de novo in two transplantable hepatomas, 7777 and 96188, has been studied by comparison of the acetyl coenzyme A carboxylase and fatty acid synthetase activities of tumor and host liver, both in animals fasted 48 hours and in those subsequently refed a fat-free diet for 48 hours. Neither acetyl-CoA carboxylase nor fatty acid synthetase of these hep...