Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants.

One of the central the mechanisms used by many soil bacteria to directly promote plant growth is the production of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. This enzyme facilitates plant growth as a consequence of the fact that it sequesters and cleaves plantproduced ACC (the immediate precursor of ethylene in plants), thereby lowering the level of ethylene in the plant. In ...

Agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. This ability has been utilized for plant genetic engineering. However, the efficiency is not sufficient for all plant species. Several studies have shown that ethylene decreased the Agrobacterium-mediated transformation frequency. Thus, A. tumefaciens with an ability to suppress ethylene evolution would increas...

The plant growth promoting strains isolated from tannery sludge and charaeteri/ed is Pseudomonas strain. The plant growth promoting Pseudomonas pittidu can utilize 1-aniinocyclopropanc-l-carboxylase (ACC) as a sole nitrogen source because it possess the unusual enzyme ACC dcaminase, which hydroly/es ACC to ammonia and a-ketobutyrate. This enzyme which is though to be intimately involved in the ...

Every minute, the world’s population grows, and in order to feed them, crop output agricultural productivity must be improved by adding crucial microorganisms that boost plant yields various ways through nitrogen fixation, secretion of both growth regulators 1-aminocyclopropane 1-carboxylate deaminase, as well some antimicrobial agents. Numerous endophytic bacteria have recently been used incre...

BACKGROUND All plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth. Changes in plant growth frequently reflect alterations in phytohormone homoeostasis by plant-growth-promoting (PGP) rhizobacteria which can decrease ethylene (ET) levels enzymatically by 1-aminocyclopropane-1-carboxylate (ACC) deaminase or produce indole acetic acid (...

Salinity is a major abiotic stress that reduces crop productivity in arid and semiarid soils. About 25% of the country's arable land is affected by different levels of salt. A considerable part of this land is under wheat cultivation each year as the country's most important crop. ACC deaminase producing bacteria increase plant resistance to stress condition by reducing stress ethylene in a var...

Agrobacterium-mediated transformation is a useful tool for the genetic modification in plants, although its efficiency is low for several plant species. Agrobacterium-mediated transformation has three major steps in laboratory-controlled experiments: the delivery of T-DNA into plant cells, the selection of transformed plant cells, and the regeneration of whole plants from the selected cells. Ea...

in this study, the ability of 32 rhizobium strains (26 strain of sinorhizobium meliloti (sm)), 4 strains of rhizobium leguminosarum biovar phaseoli (rlp) and 2 salt tolerance strains of rhizobium leguminosarum  biovar viciae (rlv) for use of acc as individual nitrogen source in rmm media was evaluated. based on the results, 25 strains were found positive for acc-deaminse, and strains according ...